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Annexin II binds progastrin and gastrin-like peptides, and mediates growth factor effects of autocrine and exogenous gastrins on colon cancer and intestinal epithelial cells

Oncogene volume 26, pages 425–440 (2007)Cite this article

Abstract

We and others have reported the presence of novel progastrin (PG)/gastrin receptors on normal and cancerous intestinal cells. We had earlier reported the presence of 33–36 kDa gastrin-binding proteins on cellular membranes of colon cancer cells. The goal of the current study was to identify the protein(s) in the 33–36 kDa band, and analyse its functional significance. A carbodiimide crosslinker was used for crosslinking radio-labeled gastrins to membrane proteins from gastrin/PG responsive cell lines. Native membrane proteins, crosslinked to the ligand, were solubulized and enriched by >1000-fold, and analysed by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry. The peptide masses were researched against the NCBInr database using the ProFound search engine. Annexin II (ANX II) was identified, and confirmed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. As HCT-116 cells express autocrine PG, the in situ association of PG with ANX II was demonstrated in pulldown assays. Direct binding of PG with ANX II was confirmed in an in vitro binding assay. In order to confirm a functional importance of these observations, sense and anti-sense (AS) ANX II RNA-expressing clones of intestinal epithelial (IEC-18) and human colon cancer (HCT-116) cell lines were generated. AS clones demonstrated a significant loss in the growth response to exogenous (IEC-18) and autocrine (HCT-116) PG. We have thus discovered that membrane-associated ANX II binds PG/gastrins, and partially mediates growth factor effects of the peptides.

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Abbreviations

Abs:

antibodies

ANX II:

Annexin II

BSA:

bovine serum albumin

CCK2R:

cholecystokinin type 2 receptor

CCK:

choecystokinin octapeptide

CRC:

colorectal cancer

DSS:

disuccinimidyl suberate

EDC:

carbodiimide crosslinker

G17:

gastrin 1–17

MALDI-TOF-MS:

matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

M r :

molecular mass

rhPG:

recombinant human progastrin

SELDI-TOF-MS:

surface-enhanced laser desorption/ionization-time of flight-mass spectrometry

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Acknowledgements

This work was supported by Grant #s CA097959 and CA72992 from the National Cancer Institute to P Singh.

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Authors and Affiliations

  1. Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, USA

    P Singh, H Wu & A Owlia

  2. Ciphergen Biosystems, Inc., Fremont, CA, USA

    C Clark

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  1. P Singh
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  2. H Wu
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  4. A Owlia
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Correspondence to P Singh.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Singh, P., Wu, H., Clark, C. et al. Annexin II binds progastrin and gastrin-like peptides, and mediates growth factor effects of autocrine and exogenous gastrins on colon cancer and intestinal epithelial cells. Oncogene 26, 425–440 (2007). https://doi.org/10.1038/sj.onc.1209798

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