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Androgen receptor levels are increased by interferons in human prostate stromal and epithelial cells

Oncogene volume 25, pages 2812–2817 (2006)Cite this article

Abstract

Proliferation of normal and malignant prostate epithelium is regulated by androgen stimulation via both the androgen receptor (AR)-positive stromal and epithelial cells. However, it is not known how AR expression is regulated in human prostate cells. We report that treatment of normal human prostate stromal cells (PrSCs) with type I IFN (alpha or beta), but not type II IFN (gamma), resulted in increased levels of AR protein. The maximal increase in AR protein levels was dependent on the dose and the duration of the IFN-alpha treatment. We found that the increase in AR protein levels was independent of de novo transcription and protein synthesis. Interestingly, the IFN-alpha treatment of PrSCs resulted in considerable nuclear accumulation of AR, stimulation of AR-mediated transcription of reporter genes, and retardation of cell proliferation. Furthermore, treatment of normal human prostate epithelial cells with IFNs (alpha, beta or gamma) also resulted in increased levels of AR protein. Together, our observations identify the androgen receptor as an IFN-regulated protein in normal human prostate stromal and epithelial cells and predict that IFN-induced levels of AR in prostate cells contribute to the regulation of androgen signaling.

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Acknowledgements

We thank Dr N Weigel and L Shemshedini for reagents. An award from the Department of Veteran Affairs (Medical Research & Development Service) to DC supported this research. The Kimmel Research Scholarship Foundation supported ZB and SCC.

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Authors and Affiliations

  1. Department of Urology, Loyola University Chicago, IL, USA

    Z Basrawala, S C Campbell & R C Flanigan

  2. Department of Radiation Oncology, Loyola University Chicago and Edward Hines Jr VA Hospital, Hines, IL, USA

    F Alimirah, H Xin, N Mohideen & D Choubey

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  1. Z Basrawala
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  2. F Alimirah
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  3. H Xin
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  4. N Mohideen
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  5. S C Campbell
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  6. R C Flanigan
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  7. D Choubey
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Correspondence to D Choubey.

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Basrawala, Z., Alimirah, F., Xin, H. et al. Androgen receptor levels are increased by interferons in human prostate stromal and epithelial cells. Oncogene 25, 2812–2817 (2006). https://doi.org/10.1038/sj.onc.1209304

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