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An unexpected role for FosB in activation-induced cell death of T cells

Oncogene volume 22, pages 1333–1339 (2003)Cite this article

Abstract

The CD95 (APO-1/Fas) system plays a major role in induction of apoptosis in lymphoid and nonlymphoid tissues. The CD95 (APO-1/Fas) ligand (CD95L) is induced in response to a variety of signals including TCR/CD3 stimulation or application of chemotherapeutic drugs. Here we report that an AP-1 site located in the 5′ untranslated region of the CD95L gene is required for TCR/CD3-mediated induction of the human CD95L promoter. Electrophoretic mobility shift assays using nuclear extracts of Jurkat T cells as well as TCR/CD3-restimulated primary human T cells demonstrated specific binding of AP-1, predominantly composed of c-Jun and FosB, to this sequence. Ectopic expression of transdominant negative Jun mutants strongly reduced CD95L promoter activity and activation-induced cell death (AICD), confirming the functional significance of FosB/c-Jun binding. Thus, our results demonstrate an important novel function for FosB dimerized with c-Jun in TCR/CD3-mediated AICD in human T cells.

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References

  • Andrews NC and Faller DV . (1991). Nucleic Acids Res, 19, 2499.

  • Chang L and Karin M . (2001). Nature, 410, 37–40.

  • Chow WA, Fang JJ and Yee JK . (2000). J. Immunol., 164, 3512–3518.

  • Cockerill GW, Bert AG, Ryan GR, Gamble JR, Vadas MA and Cockerill PN . (1995). Blood, 86, 2689–2698.

  • Crabtree GR . (1999). Cell, 96, 611–614.

  • Dhein J, Walczak H, Baumler C, Debatin KM and Krammer PH . (1995). Nature, 373, 438–441.

  • Eichhorst ST, Muller M, Li-Weber M, Schulze-Bergkamen H, Angel P and Krammer PH . (2000). Mol. Cell Biol., 20, 7826–7837.

  • Faris M, Kokot N, Latinis K, Kasibhatla S, Green DR, Koretzky GA and Nel A . (1998). J. Immunol., 160, 134–144.

  • Friesen C, Herr I, Krammer PH and Debatin KM . (1996). Nat. Med., 2, 574–577.

  • Hafezi F, Steinbach JP, Marti A, Munz K, Wang ZQ, Wagner EF, Aguzzi A and Reme CE . (1997). Nat. Med., 3, 346–349.

  • Harwood FG, Kasibhatla S, Petak I, Vernes R, Green DR and Houghton JA . (2000). J. Biol. Chem., 275, 10023–10029.

  • Holtz-Heppelmann CJ, Algeciras A, Badley AD and Paya CV . (1998). J. Biol. Chem., 273, 4416–4423.

  • Ivanov VN and Nikolic-Zugic J . (1997). J. Biol. Chem., 272, 8558–8566.

  • Karin M . (1995). J. Biol. Chem., 270, 16483–16486.

  • Kasibhatla S, Brunner T, Genestier L, Echeverri F, Mahboubi A and Green DR . (1998). Mol. Cell, 1, 543–551.

  • Kasibhatla S, Genestier L and Green DR . (1999). J. Biol. Chem., 274, 987–992.

  • Kirchhoff S, Muller WW, Krueger A, Schmitz I and Krammer PH . (2000). J. Immunol., 165, 6293–6300.

  • Kolbus A, Herr I, Schreiber M, Debatin KM, Wagner EF and Angel P . (2000). Mol. Cell Biol., 20, 575–582.

  • Lidwell K and Griffiths R . (2000). J. Neurosci. Res., 62, 427–439.

  • Li-Weber M, Laur O, Dern K and Krammer PH . (2000). Eur. J. Immunol., 30, 661–670.

  • Li-Weber M, Laur O, Hekele A, Coy J, Walczak H and Krammer PH . (1998). Eur. J. Immunol., 28, 2373–2383.

  • Li-Weber M, Laur O and Krammer PH . (1999). Eur. J. Immunol., 29, 3017–3027.

  • Macian F, Garcia-Rodriguez C and Rao A . (2000). EMBO J., 19, 4783–4795.

  • Matsui K, Xiao S, Fine A and Ju ST . (2000). J. Immunol., 164, 3002–3008.

  • Mittelstadt PR and Ashwell JD . (1998). Mol. Cell Biol., 18, 3744–3751.

  • Mittelstadt PR and Ashwell JD . (1999). J. Biol. Chem., 274, 3222–3227.

  • Norian LA, Latinis KM and Koretzky GA . (1998). J. Immunol., 161, 1078–1082.

  • Passegue E and Wagner EF . (2000). EMBO J., 19, 2969–2979.

  • Rao A, Luo C and Hogan PG . (1997). Annu. Rev. Immunol., 15, 707–747.

  • Rivera I, Harhaj EW and Sun SC . (1998). J. Biol. Chem., 273, 22382–22388.

  • Rooney JW, Hoey T and Glimcher LH . (1995). Immunity, 2, 473–483.

  • Schmitz I, Kirchhoff S and Krammer PH . (2000). Int. J. Biochem. Cell Biol., 32, 1123–1136.

  • Stein B, Baldwin. Jr., AS, Ballard DW, Greene WC, Angel P and Herrlich P . (1993). EMBO J., 12, 3879–3891.

  • Su B, Jacinto E, Hibi M, Kallunki T, Karin M and Ben-Neriah Y . (1994). Cell, 77, 727–736.

  • Weitzman JB, Fierte L, Matsuo K and Yaniv M . (2000). Mol. Cell, 6, 1109–1119.

  • Yamamura Y, Hua X, Bergelson S and Lodish HF . (2000). J. Biol. Chem., 275, 36295–36302.

  • Zhang J, Gao JX, Salojin K, Shao Q, Grattan M, Meagher C, Laird DW and Delovitch TL . (2000). J. Exp. Med., 191, 1017–1030.

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Acknowledgements

We are grateful to Pola Linzmayer, Wolfgang W Müller, Christine Stumpf and Simone Stößer for expert technical assistance. We thank Drs Weigand and Sebens for taking blood samples. We also thank all our blood donors.

This work was supported by grants from Sonderforschungsbereich der deutschen Forschungsgemeinschaft SFB 601 and 405, Deutsches Krebsforschungszentrum/Israeli Minister of Science (DKFZ/MOS) (Ca86), Deutsch-Israelische Projektkooperation (DIP), and the Sander Stiftung.

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Authors and Affiliations

  1. Tumor Immunology Program, Heidelberg, 69120, Germany

    Sven Baumann, Sören T Eichhorst, Andreas Krueger, Peter H Krammer & Sabine Kirchhoff

  2. SignaI Transduction and Growth Control, German Cancer Research Center, Heidelberg, 69120, Germany

    Jochen Hess & Peter Angel

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  1. Sven Baumann
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Correspondence to Peter H Krammer.

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Baumann, S., Hess, J., Eichhorst, S. et al. An unexpected role for FosB in activation-induced cell death of T cells. Oncogene 22, 1333–1339 (2003). https://doi.org/10.1038/sj.onc.1206126

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