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The ZYG-1 kinase, a mitotic and meiotic regulator of centriole replication

Oncogene volume 21, pages 6201–6208 (2002)Cite this article

As the primary microtubule-organizing center (MTOC) of the cell, the centrosome is a major structural determinant of the mitotic spindle. Like each chromosome, the centrosome must replicate once and only once per cell cycle in order to maintain the fidelity of nuclear division and the integrity of the genome. While the mechanisms that govern this process are not well understood and are sure to be complex, centrosome duplication can be described as involving three general steps. First, the centrosome splits to form two centrosomes, each with half the reproductive capacity of the original. Second, a replication step restores each new centrosome to full reproductive capacity. Third, the replicated centrosomes sever any remaining connections and move apart. In the past few years, several factors have been identified that regulate each of these steps. Among these factors is the ZYG-1 kinase of C. elegans, which functions during both mitotic and meiotic divisions (O'Connell et al., 2001).

The centrosome is a non-membrane bound organelle composed of two distinct domains. At the center is a pair of centrioles, cylindrical structures composed of microtubules. While the microtubules of the centriole cylinder most often are arranged as nine sets of triplets, significant deviations from this basic structure have been observed (Phillips, 1967). This includes early Drosophila and C. elegans embryos which have centrioles composed of nine singlet or nine doublet microtubules (Wolf et al., 1978; Moritz et al., 1995). A centriole pair contains one mature or mother centriole, at least 1.5-cell generations old, and one immature or daughter centriole, produced during the preceding S phase. Prior to initiating duplication, the daughter is aligned perpendicular to the mother so that its long axis intersects that of the mother. The centrioles are tightly associated with the reproductive capacity of the centrosome (Sluder and Rieder, 1985) and have been implicated in organizing the PCM into a discrete focus (Bobinnec et al., 1998). Surrounding the centrioles is the pericentriolar material or PCM, a complex and dynamic meshwork of proteins involved in microtubule nucleation (Gould and Borisy, 1977).

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Acknowledgements

I wish to thank Marie Delattre and Pierre Gönczy for sharing unpublished data and Andy Golden for critical reading of the manuscript. This work was supported by a grant from the National Institutes of Health Intramural funds.

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  1. Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 2A07, Bethesda, 20892, Maryland, MD, USA

    Kevin F O'Connell

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Correspondence to Kevin F O'Connell.

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O'Connell, K. The ZYG-1 kinase, a mitotic and meiotic regulator of centriole replication. Oncogene 21, 6201–6208 (2002). https://doi.org/10.1038/sj.onc.1205776

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