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  • Oncogenomics
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Cloning and characterization of PAK5, a novel member of mammalianp21-activated kinase-II subfamily that is predominantly expressed in brain

Oncogene volume 21, pages 3939–3948 (2002)Cite this article

Abstract

The p21-activated kinase (PAK) family of protein kinases has recently attracted considerable attention as an effector of Rho family of small G proteins and as an upstream regulator of MAPK signalling pathways during cellular events such as re-arrangement of the cytoskeleton and apoptosis. We have cloned a novel human PAK family kinase that has been designated as PAK5. PAK5 contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 is structurally most related to PAK4 and PAK6 to make up the PAK-II subfamily. We have shown that PAK5 preferentially binds to CDC42 in the presence of GTP and that CRIB motif is essential for this interaction. PAK5 is a functional protein kinase but unlike PAK-I family kinases (PAK1, 2, and 3), the kinase activity of PAK5 does not seem to require the binding of CDC42. Overexpression of PAK5 activates the JNK kinase pathway but not p38 or ERK pathways. PAK5 transcript is predominantly expressed in brain as revealed by Northern blot and in situ hybridization. The expression pattern of PAK5 is distinct from that of PAK4 and PAK6, suggesting a functional division among PAK-II subfamily kinases based on differential tissue distribution.

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Acknowledgements

Work at the Center for Experimental Bioinformatics was supported by a generous grant from the Danish National Research Foundation. A Pandey was supported by a Howard Temin Award from the National Cancer Institute (CA 75447) and by a travel award from the Plasmid Foundation, Roskilde, Denmark.

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Author notes

  1. Akhilesh Pandey and Ippeita Dan: A Pandey and I Dan contributed equally to this work

Authors and Affiliations

  1. Center for Experimental Bioinformatics, University of Southern Denmark, Campusvej 55, DK-5230, Odense M, Denmark

    Akhilesh Pandey, Troels Z Kristiansen, Jesper Voldby, Blagoy Blagoev & Matthias Mann

  2. Department of Biological Science, Kusumi Membrane Organizer Project, ERATO, JST, Graduate School of Science, Nagoya University, Chikusa-ku, 464-8602, Nagoya, Japan

    Ippeita Dan, Norinobu M Watanabe & Eriko Kajikawa

  3. Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical Center, Boston, 02115, Massachusetts, MA, USA

    Roya Khosravi-Far

Authors
  1. Akhilesh Pandey
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  2. Ippeita Dan
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  9. Matthias Mann
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Corresponding authors

Correspondence to Akhilesh Pandey, Ippeita Dan or Matthias Mann.

Additional information

The human PAK5 cDNA sequence has been submitted to DDBJ/EMBL/GenBank (accession no. AB040812).

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Pandey, A., Dan, I., Kristiansen, T. et al. Cloning and characterization of PAK5, a novel member of mammalianp21-activated kinase-II subfamily that is predominantly expressed in brain. Oncogene 21, 3939–3948 (2002). https://doi.org/10.1038/sj.onc.1205478

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