Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

v-Abl utilizes multiple mechanisms to drive G1/S progression in fibroblasts

Oncogene volume 19, pages 801–809 (2000)Cite this article

Abstract

Transformation of 3T3 fibroblasts by the v-Abl tyrosine kinase replaces mitogenic and adhesion signals normally required for cell cycle progression. A 3T3 cell line conditionally transformed with v-Abl has been used to study v-Abl’s effects on cell cycle in the context of either serum depletion or absence of adhesion signals. We show that E2F-dependent mRNAs, encoding proteins required for cell cycle progression, are induced by v-Abl. In addition, we identify two previously unknown targets of v-Abl signaling: (1) cyclin D1 and D2 mRNAs are induced upon v-Abl activation; and (2) the CDK inhibitor p27 is decreased upon v-Abl activation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6

Similar content being viewed by others

References

  • Aktas H, Cai H and Cooper GM. . 1997 Mol. Cell. Biol. 17: 3850–3857.

    Article  CAS  Google Scholar 

  • Alessandrini A, Chiaur DS and Pagano M. . 1997 Leukemia 11: 342–345.

    Article  CAS  Google Scholar 

  • Assoian RK and Zhu X. . 1997 Curr. Opin. Cell Biol. 9: 93–98.

  • Ausubel FM. . 1987 Current Protocols in Molecular Biology. Green Publication Associates and Wiley-Interscience: J Wiley, New York.

    Google Scholar 

  • Birchenall-Roberts MC, Yoo YD, Bertolette DC 3rd, Lee KH, Turley JM, Bang OS, Ruscetti FW and Kim SJ. . 1997 J. Biol. Chem. 272: 8905–8911.

    Article  CAS  Google Scholar 

  • Blain SW, Montalvo E and Massague J. . 1997 J. Biol. Chem. 272: 25863–25872.

    Article  CAS  Google Scholar 

  • Brooks AR, Shiffman D, Chan CS, Brooks EE and Milner PG. . 1996 J. Biol. Chem. 271: 9090–9099.

    Article  CAS  Google Scholar 

  • Chen CA and Okayama H. . 1988 Biotechniques 6: 632–638.

  • Chen YY and Rosenberg N. . 1992 Proc. Natl. Acad. Sci. USA 89: 6683–6687.

  • Cleveland J, Dean M, Rosenberg N, Wang J and Rapp U. . 1989 Mol. Cell. Biol. 9: 5685–5695.

    Article  CAS  Google Scholar 

  • Coats S, Flanagan WM, Nourse J and Roberts JM. . 1996 Science 272: 877–880.

    Article  CAS  Google Scholar 

  • Danial NN, Losman JA, Lu T, Yip N, Krishnan K, Krolewski J, Goff SP, Wang JYJ and Rothman PB. . 1998 Mol. Cell. Biol. 18: 6795–6804.

    Article  CAS  Google Scholar 

  • Danial NN, Pernis A and Rothman PB. . 1995 Science 269: 1875–1877.

    Article  CAS  Google Scholar 

  • DelSal G, Loda M and Pagano M. . 1996 Crit. Rev. Oncog. 7: 127–142.

  • Dyson N. . 1998 Genes Dev. 12: 2245–2262.

  • Fang F, Orend G, Watanabe N, Hunter T and Ruoslahti E. . 1996 Science 271: 499–502.

    Article  CAS  Google Scholar 

  • Firpo EJ, Koff A, Solomon MJ and Roberts JM. . 1994 Mol. Cell. Biol. 14: 4889–4901.

    Article  CAS  Google Scholar 

  • Harper JW. . 1997 Cancer Surv. 29: 91–107.

  • Kato JY, Matsuoka M, Polyak K, Massague J and Sherr CJ. . 1994 Cell 79: 487–496.

    Article  CAS  Google Scholar 

  • Lavoie JN, L’Allemain G, Brunet A, Muller R and Pouyssegur J. . 1996 J. Biol. Chem. 271: 20608–20616.

    Article  CAS  Google Scholar 

  • Matsumura I, Kitamura T, Wakao H, Tanaka H, Hashimoto K, Albanese C, Downward J, Pestell RG and Kanakura Y. . 1999 EMBO J. 18: 1367–1377.

  • Morgan DO. . 1995 Nature 374: 131–134.

    Article  CAS  Google Scholar 

  • Muller D, Bouchard C, Rudolph B, Steiner P, Stuckmann I, Saffrich R, Ansorge W, Huttner W and Eilers M. . 1997 Oncogene 15: 2561–2576.

    Article  Google Scholar 

  • Nguyen H, Gitig DM and Koff A. . 1999 Mol. Cell Biol. 19: 1190–1201.

  • Nourse J, Firpo E, Flanagan WM, Coats S, Polyak K, Lee MH, Massague J, Crabtree GR and Roberts JM. . 1994 Nature 372: 570–573.

    Article  CAS  Google Scholar 

  • Phelps DE and Xiong Y. . 1997 Methods Enzymol. 283: 194–205.

  • Polyak K, Kato JY, Solomon MJ, Sherr CJ, Massague J, Roberts JM and Koff A. . 1994 Genes Dev. 8: 9–22.

  • Puissant C and Houdebine LM. . 1990 Biotechniques 8: 148–149.

  • Quelle DE, Ashmun RA, Hannon GJ, Rehberger PA, Trono D, Richter KH, Walker C, Beach D, Sherr CJ and Serrano M. . 1995 Oncogene 11: 635–645.

  • Renshaw MW, Kipreos ET, Albrecht MR and Wang JY. . 1992 EMBO J. 11: 3941–3951.

  • Reynisdottir I, Polyak K, Iavarone A and Massague J. . 1995 Genes Dev. 9: 1831–1845.

  • Riggs KJ, Saleque S, Wong KK, Merrell KT, Lee JS, Shi Y and Calame K. . 1993 Mol. Cell. Biol. 13: 7487–7495.

    Article  CAS  Google Scholar 

  • Sheaff RJ. . 1997 Methods Enzymol. 283: 173–193.

  • Sherr CJ. . 1996 Science 274: 1672–1677.

    Article  CAS  Google Scholar 

  • Sherr CJ and Roberts JM. . 1995 Genes Dev. 9: 1149–1163.

  • Slansky JE, Li Y, Kaelin WG and Farnham PJ. . 1993 Mol. Cell Biol. 13: 1610–1618.

  • Takuwa N and Takuwa Y. . 1997 Mol. Cell. Biol. 17: 5348–5358.

    Article  CAS  Google Scholar 

  • Vlach J, Hennecke S, Alevizopoulos K, Conti D and Amati B. . 1996 EMBO J. 15: 6595–6604.

  • Weissinger EM, Eissner G, Grammer C, Fackler S, Haefner B, Yoon LS, Lu KS, Bazarov A, Sedivy JM, Mischak H and Kolch W. . 1997 Mol. Cell Biol. 17: 3229–3241.

  • Wong KK, Zou X, Merrell KT, Patel AJ, Marcu KB, Chellappan S and Calame K. . 1995 Mol. Cell Biol. 15: 6535–6544.

  • Zhu X, Ohtsubo M, Bohmer RM, Roberts JM and Assoian RK. . 1996 J. Cell Biol. 133: 391–403.

  • Zou X, Rudchenko S, Wong K and Calame K. . 1997 Genes Dev. 11: 654–662.

Download references

Acknowledgements

We thank Dr S Goff for critically reading this manuscript and members of the Calame and Goff laboratories for helpful discussions. We would like to thank the following colleagues for their generous gifts of murine cDNAs: Dr David Cobrinik for cyclin E, Dr Berthold Henglein for cyclin A, and Dr Lars Thelander for ribonucleotide reductase. We would also like to thank Drs Jill Slansky and Peggy Farnham for the DHFR wild type and mutant promoter plasmids.

Author information

Authors and Affiliations

  1. Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, HHSC 1202, New York, 10032, NY, USA

    Margaret Coutts & Kathryn Calame

  2. Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, 701 West 168th Street, HHSC 1202, New York, 10032, NY, USA

    Xiaoming Zou & Kathryn Calame

Authors
  1. Margaret Coutts
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaoming Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kathryn Calame
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Coutts, M., Zou, X. & Calame, K. v-Abl utilizes multiple mechanisms to drive G1/S progression in fibroblasts. Oncogene 19, 801–809 (2000). https://doi.org/10.1038/sj.onc.1203398

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1203398

Keywords

This article is cited by

Search

Advanced search

Quick links