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  • Original Paper
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The extracellular-signal-regulated protein kinases (Erks) are required for UV-induced AP-1 activation in JB6 cells

Oncogene volume 18, pages 2828–2835 (1999)Cite this article

Abstract

Mitogen activated protein (MAP) kinase belongs to a large family of serine/threonine protein kinases, including extracellular-signal-regulated protein kinases (Erks), P38 kinase and c-Jun N-terminal kinases (JNKs). Although previous work has shown that both Erks and JNKs are activated in cells in response to ultraviolet (UV) irradiation, most studies have focused only on the role of JNKs in UV-induced AP-1 activation. Hence, the role of Erks in UV-induced AP-1 activity is not well defined. We here have investigated this issue by using MAP kinase kinase (MEK1) inhibitor PD098059 and a dominant negative Erk2, as well as wild-type Erk2, in a JB6 cell model. PD098059 inhibited UVB- or UVC-induced AP-1 activity and phosphorylation of MEK1 and Erks, but not JNKs, in JB6 Cl 41 cells. Overexpression of wild-type Erk2 in Cl 30.7b cells that contain small amounts of Erks caused a 46.6- or 138.1-fold increase of AP-1 activity by UVB and UVC, respectively; introduction of a dominant negative Erk2 into Cl 41 cells significantly blocked the UV-induced Erks activation as well as the AP-1 activation. In contrast, overexpression of wild-type Erk2 in Cl 30.7b cells and dominant negative Erk2 in Cl 41 cells did not show a marked influence on the phosphorylation of JNKs. These results demonstrate that activation of Erks, in addition to the previously reported JNKs, is required for UV-induced AP-1 activation.

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Abbreviations

UV:

ultraviolet

AP-1:

activated protein-1

EGF:

epidermal growth factor

Erk:

extracellular signal-regulated protein kinase

MAP kinase:

mitogen-activated protein kinase

JNK:

c-Jun N-terminal kinase

P+:

tumor promotion-sensitive

P−:

tumor promotion-resistant

TPA:

12-O-tetradecanoyl phorbol-13-acetate

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Acknowledgements

We thank Drs HHO Schmid and Douglas Bibus for their critical reading and Ms Jeanne Ruble for secretarial assistance. This work was supported in part by the Hormel Foundation.

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  1. Unité de Recombinaison et Expression Génétique (INSERM U163), Institut Pasteur, Paris, France

    Chuanshu Huang, Wei-ya Ma & Zigang Dong

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Huang, C., Ma, Wy. & Dong, Z. The extracellular-signal-regulated protein kinases (Erks) are required for UV-induced AP-1 activation in JB6 cells. Oncogene 18, 2828–2835 (1999). https://doi.org/10.1038/sj.onc.1202639

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