Abstract
Utilizing the cytoplasmic tail of Transforming Growth Factor Receptor Type II (TGFβ RII) as bait in a yeast two hybrid system, we have identified human cyclin B2 as a direct physical partner of TGFβ RII. Analysis of deletion mutants of glutathione-S-transferase (GST)-cyclin B2 mapped its binding domain for TGFβ RII to the C-terminal and revealed a negative regulatory region immediately upstream of the cyclin box. Using recombinant proteins, Cdc2 was demonstrated to indirectly interact with TGFβ RII via cyclin B2. This interaction was reproduced in THP-1 monocytic cells, where TGFβ treatment markedly enhanced the ability of cyclin B2 and, correspondingly, Cdc2 from TGFβ-treated THP-l cells, to bind the GST-TGFβ RII fusion protein. More importantly, TGFβ RII co-precipitated with cyclin B2 in TGFβ-treated THP-1 cells. TGFβ treatment also caused threonine phosphorylation of Cdc2 in the TGFβ RII-cyclin B2-Cdc2 complex in THP1 cells, in parallel with down regulation of Cdc2 function as measured by histone H1 kinase activity. Cyclin B1 had the same capacity to bind TGFβ RII and mediate indirect Cdc2 binding. These results suggest an alternative mechanism that cell cycle arrest in the G1/S phase caused by TGFβ may, in part, be due to inactivation of cyclin B/Cdc2 kinase, which is needed for entry into the G2/M phase.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA and Struhl K. . 1995 Current Protocols in Molecular Biology. John Wiley and Sons, Inc: New York, NY. pp. 18.0.1–18.4.7.
Blain SW, Montalvo E and Massague J. (1997). . J. Biol. Chem. 272: 25863–25872.
Clarke PR. . 1994 Cur. Biol. 5: 40–42.
Chen RH and Derynck R. . 1994 J. Biol. Chem. 269: 22868–22874.
Chen RH, Miettinen PJ, Maruoka EM, Choy L and Derynck R. . 1995a Nature 377: 548–552.
Chen RH, Moses HL, Maruoka E, Derynck R and Kawabata M. . 1995b J. Biol. Chem. 270: 12235–12241.
Chen RH, Ebner R and Derynck R. . 1993 Science 260: 1335–1338.
Chen YG, Liu F and Massague J. . 1997 EMBO J. 16: 3866–3876.
Chomczynski P and Sacchi N. . 1987 Ana. Biochem. 162: 156–159.
Derynck R. . 1994 TIBS 19: 548–553.
Enoch T and Nurse P. . (1990) Cell 60: 665–673
Ewen ME, Sluss HK, Whitehouse LL and Livingston DM. ( . 1993 Cell 74: 1009–1020.
Fields S and Song O. . 1989 Nature 340: 245–247.
Ghiara JB, Richardson HE, Sugimoto K, Henze M, Lew DJ, Wittenberg C and Reed SI. . 1991 Cell 65: 163–174.
Goodrich DW, Wang NP, Qian YW, Lee EY and Lee WH. . 1991 Cell 67: 293–302.
Gould KL, Moreno S, Owen DJ, Sazer S, and Nurse P. . 1991 EMBO J. 10: 3297–3309.
Guarente L. . 1993 Proc. Natl. Acad. Sci. USA 90: 1639–1641.
Harper JW, Adami GR, Wei N, Keyomarsi K and Elledge SJ. . 1993 Cell 75: 805–816.
Jackman M, Firth M and Pines J. . 1995 EMBO J. 14: 1646–1654.
Koff A, Ohtsuki M, Polyak K, Roberts JM and Massague J. . 1993 Science 260: 536–539.
Kretzschmar M, Doody J and Massague J. . 1997 Nature 389: 618–622.
Laiho M, DeCaprio A, Ludlow JW, Livingston DM and Massague J. . 1990 Cell 62: 175–185.
Lin HY, Wang XF, Ng-Eaton E, Weinberg RA and Lodish HF. . 1992 Cell 68: 775–785.
Liu F, Pouponnot C and Massague J. . 1997 Genes Dev. 11: 3157–3167.
Lock LF and Wickramasinghe D. . 1994 Treads in Cell Biol. 4: 404–405.
Macias-Silva M, Abdollah S, Hoodless PA, Pirone R, Attisano L, and Wrana JL. . 1996 Cell 87: 1215–1224.
Massague J, Hata A and Liu F. . 1997 Trends Cell Biol. 7: 187–192.
McGowan CH and Russell P. . 1995 EMBO J. 14: 2166–2175.
Miyazono K. Dijke T, Ichijo H and Helden CH. . 1994 Adv. Immunol. 55: 181
Mueller PR, Coleman TR and Dunphy WG. . 1995 Science 270: 86–90.
Parker LL and Piwnica-Worms H. . 1992 Science 257: 1955–1957.
Pines J and Hunter TH. . 1989 Cell 58: 833–846.
Ravitz RJ, Yan S, Herr KD and Wenner CE. (1995). . Cancer Res. 55: 1413–1416.
Reynisdottir I and Massague J. . 1997 Genes Dev. 11: 492–503.
Surana U, Robitch H, Price C, Schuster T, Fitch I, Futcher AB and Nasmyth K. . 1991 Cell 65: 145–161.
Toyoshima H and Hunter T. . 1994 Cell 78: 67–74.
Wang T, Donahoe PK and Zervos AS. . 1994 Science 265: 674–676.
Wang T, Danielson PD, Li BY, Shah PC, Kim SD and Donahoe PK. . 1996 Science 271: 1120–1122.
Wrana JL, Attisano l, Wieser R, Ventura F and Massague J. . 1994 Nature 370: 341–347.
Acknowledgements
We are grateful to Dr X-F Wang (Duke University Medical Center, Durham, NC, USA) for the TGFβ receptor type II plasmid, Dr Steven I Reed (Scripps Research Institute, La Jolla, CA, USA) for full length cyclin B2, and Dr Tony Hunter (The Salk Institute for Biological Studies, San Diego, CA, USA) for cyclin B1 and Cdc2 plasmids.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, J., Wei, S., Burnette, P. et al. Functional association of TGF-β receptor II with cyclin B. Oncogene 18, 269–275 (1999). https://doi.org/10.1038/sj.onc.1202263
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1202263
Keywords
This article is cited by
-
The effects of Di-2-ethylhexyl phthalates (DEHP) on the cell cycle of the endometrial cancer cell lines (ECC-1)
Toxicology and Environmental Health Sciences (2014)
-
Strong expression of cyclin B2 mRNA correlates with a poor prognosis in patients with non-small cell lung cancer
Tumor Biology (2014)
-
Genetics of breast cancer bone metastasis: a sequential multistep pattern
Clinical & Experimental Metastasis (2014)
-
Gene network analysis on the effect of Viscum album var. coloratum in T cells stimulated with anti-CD3/CD28 antibodies
Archives of Pharmacal Research (2011)
-
In vitro nuclear interactome of the HIV-1 Tat protein
Retrovirology (2009)