Abstract
The contribution of the ETS2 transcription factor to the transformed state in prostate cancer cells has been assessed. Northern blot analysis easily detects ETS2 in DU145 and PC3, high grade human prostate cell lines, but ETS2 is not present in lower grade LNCaP cells. Stable transfection of PC3 and DU145 prostate cell lines with an antisense ETS2 vector or with a dominant negative ETS2 mutant significantly reduced the ability of DU145 and PC3 cells to form large colonies in soft agar. Thus, the presence of ETS2 is positively correlated with a more transformed phenotype and blockage of ETS2 function can reduce transformed properties of prostate cancer cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sementchenko, V., Schweinfest, C., Papas, T. et al. ETS2 function is required to maintain the transformed state of human prostate cancer cells. Oncogene 17, 2883–2888 (1998). https://doi.org/10.1038/sj.onc.1202220
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1202220
Keywords
This article is cited by
-
Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods
Journal of Translational Medicine (2015)
-
Ets-1 global gene expression profile reveals associations with metabolism and oxidative stress in ovarian and breast cancers
Cancer & Metabolism (2013)
-
Elf5 inhibits the epithelial–mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2
Nature Cell Biology (2012)
-
The role of the proto-oncogene ETS2 in acute megakaryocytic leukemia biology and therapy
Leukemia (2008)
-
Frequent overexpression of ETS-related gene-1 (ERG1) in prostate cancer transcriptome
Oncogene (2005)