Abstract
LONDON. Royal Microscopical Society, May 21.—Mr. A. Chaston Chapman, president, in the chair.—E. W. Bowell: Brief observations on the mounting and photomicrography of radulae. Glycerin jelly preparations are only suitable for large objects, and the actual size of the radute which most need illustrating is exceedingly small compared to the size of the large pectinibranchs of which the appearance is familiar from text-book illustrations; the details which require figuring are often of the same order of size as bacteria. The only satisfactory way to deal with such objects is to stain them and mount them in Canada balsam or other normal medium.—R. J. Ludford: Experiments on the impregnation of the Golgi apparatus by means of osmium tetroxide. When small pieces of transplantable tumours, after fixation in Mann's corrosive osmic solution, are transferred to 2 per cent, osmic acid solution at various temperatures above room temperature, marked changes are subsequently seen in the structure of the cells. The following is the general sequence of changes observed: (i.) the Golgi apparatus first appears as granules or faint rodlets; (ii.) the rodlets become thicker and the cytoplasm commences to shrink; (iii.) the rodlets appear to be anastomosed to form a network, and the ground cytoplasm becomes coarsely granular; (iv.) the whole of the Golgi apparatus is impregnated deep black, and the ground cytoplasm is distorted so as to give the appearance of a tangled network, or reticulate structure, and there is considerable non-specific reduction of the osmic acid; (v.) thereafter, the cell becomes more and more deeply osmicated, until the cytoplasm appears homogeneously black. From the point of view of these changes, the Golgi apparatus is that region of the cytoplasm which brings about the reduction of osmium tetroxide at a lower temperature, or in a shorter time, than is required to produce a total blackening of the cell.
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Societies and Academies. Nature 113, 913–916 (1924). https://doi.org/10.1038/113913b0
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DOI: https://doi.org/10.1038/113913b0