Abstract
LAST year when Mr. Atkins and I were searching for a method of extracting sap unchanged from various vegetable tissues, treatment with liquid air suggested itself and proved a valuable means for attaining this object. The rapidity of its action in suspending vital processes and chemical changes and in rendering protoplasm permeable, suggested its further application as a fixative. Since then most promising results have been obtained in various cells and tissues by Miss E. S. Marshall, working in this laboratory, showing various nuclear and cytoplasmic structures with great clearness and with a complete absence of plasmolysis.
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DIXON, H. Liquid Air as a Fixative. Nature 92, 609 (1914). https://doi.org/10.1038/092609a0
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DOI: https://doi.org/10.1038/092609a0
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