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Association analysis of vitamin D-binding protein gene polymorphisms with variations of obesity-related traits in Caucasian nuclear families

International Journal of Obesity volume 31, pages 1319–1324 (2007)Cite this article

Abstract

Background:

Vitamin D-binding protein (DBP) gene is well known for its function on glucose and vitamin D metabolism in human populations. Previous studies suggested that the in vivo DBP level may play a role in the etiology of obesity. However, few studies explored the contribution of DBP gene to the variance of obesity phenotypes.

Objective:

To investigate the relationship of DBP polymorphisms and obesity in Caucasian nuclear families.

Design:

We genotyped 14 single-nucleotide polymorphisms (SNPs) located in and around the DBP gene in 1873 Caucasian subjects from 405 nuclear families. Three obesity-related quantitative phenotypes were investigated, including body mass index (BMI), fat mass and percentage of fat mass (PFM). Single SNPs and haplotypes (three blocks) were tested by family-based association using the FBAT software.

Results:

SNP2 (rs17467825) and its corresponding haplotype GAA (frequency 0.270) in block1 showed strongest associations with PFM (P=0.0011 and 0.0023, respectively). In multivariate test significant association was also observed for SNP2 with fat_mass&BMI&PFM (P=0.0098). Subsequent sex-stratified analyses demonstrated nominal association for SNP2 and haplotype GAA with PFM in the female subgroup.

Conclusion:

Polymorphisms of DBP gene were significantly association with human PFM, especially in female, suggesting the importance of DBP gene in the pathogenesis of human obesity.

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Acknowledgements

The study was partially supported by grants from NIH (R01 AR050496, K01 AR02170-01, R01 AR45349-01 and R01 GM60402-01A1) and an LB595 grant from the State of Nebraska. This study also benefited from grants from Chinese National Science Foundation, Huo Ying Dong Education Foundation and Ministry of Education of PR China, Hunan Normal University and Xi'an Jiao Tong University.

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Author notes

  1. H Jiang and D-H Xiong: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha, Hunan, PR, China

    H Jiang, Y-F Guo, F Yang, Y Chen & H-W Deng

  2. Osteoporosis Research Center and Department of Biomedical Sciences, Creighton University, Omaha, NE, USA

    H Jiang, D-H Xiong, H Shen, P Xiao, F Yang, Y Chen, F Zhang & R R Recker

  3. The Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Molecular Genetics, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR, China

    Y-F Guo, F Zhang & H-W Deng

  4. Departments of Orthopedic Surgery and Basic Medical Sciences, University of Missouri – Kansas City, Kansas City, MO, USA

    H-W Deng

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Jiang, H., Xiong, DH., Guo, YF. et al. Association analysis of vitamin D-binding protein gene polymorphisms with variations of obesity-related traits in Caucasian nuclear families. Int J Obes 31, 1319–1324 (2007). https://doi.org/10.1038/sj.ijo.0803583

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