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Fat-specific transgenic expression of resistin in the spontaneously hypertensive rat impairs fatty acid re-esterification

International Journal of Obesity volume 30, pages 1157–1159 (2006)Cite this article

Abstract

Objective:

To investigate the mechanism by which fat-specific transgenic expression of resistin affects fatty acid metabolism in the spontaneously hypertensive rat (SHR).

Design:

Basal- and adrenaline-stimulated lipolysis, basal- and insulin-stimulated lipogenesis as well as the site (glycerol versus acyl moiety) of glucose incorporated into triglycerides were determined in adipose tissue isolated from SHR-Resistin transgenic and SHR control rats.

Results:

A moderate expression of transgenic resistin in adipose tissue was associated with significant increase in the FFA/glycerol ratio during adrenaline-stimulated lipolysis in the SHR-Resistin transgenic rats (3.27±0.26) compared to SHR controls (2.11±0.10, P=0.0005). Transgenic SHR also exhibited a significant decrease in FFA re-esterification in adipose tissue (approximately by 23%).

Conclusion:

These findings raise the possibility that the prodiabetic effects of transgenic resistin may be partly mediated by increased FFA release from adipose tissue due to impaired FFA re-esterification in adipocytes.

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Acknowledgements

This study was supported by grant 301/03/0751 from the Grant Agency of the Czech Republic to MP, by grant 1M6837805002 from the Ministry of Education of the Czech Republic to MP, grant 7403–3 from Internal Grant Agency of the Ministry of Health of the Czech Republic to LK and grants TWO1236 and HL63709 from the National Institutes of Health to TK. MP is an International Research Scholar of the Howard Hughes Medical Institute.

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Authors and Affiliations

  1. Institute of Physiology, Czech Academy of Sciences and Center for Applied Genomics, Prague, Czech Republic

    M Pravenec, V Landa, V Zídek, P Mlejnek & M šimáková

  2. Institute of Clinical and Experimental Medicine, Prague, Czech Republic

    L Kazdová & M Cahová

  3. Department of Laboratory Medicine, University of California, San Francisco, USA

    J Wang, N Qi & T W Kurtz

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  1. M Pravenec
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  2. L Kazdová
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  4. V Landa
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  5. V Zídek
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  6. P Mlejnek
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  7. M šimáková
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  8. J Wang
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  9. N Qi
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  10. T W Kurtz
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Correspondence to M Pravenec.

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Pravenec, M., Kazdová, L., Cahová, M. et al. Fat-specific transgenic expression of resistin in the spontaneously hypertensive rat impairs fatty acid re-esterification. Int J Obes 30, 1157–1159 (2006). https://doi.org/10.1038/sj.ijo.0803237

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