X-ray crystallography

Definition

X-ray crystallography is a technique that uses X-ray diffraction patterns to determine high-resolution, three-dimensional structures of molecules such as proteins, small organic molecules, and materials. The substance of interest must be in crystalline form, which typically requires testing various crystallization conditions.

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News and Comment

  • News and Views |

    New structures of the glucagon-like peptide-1 (GLP-1) and glucagon receptors in complex with peptide and nonpeptide ligands provide a comprehensive, detailed picture of the molecular mechanisms of action of family B GPCRs. This opens the door for true structure-based drug discovery aimed at both novel orthosteric and allosteric subsites of the receptors.

    • Thue W Schwartz
    •  & Thomas M Frimurer
  • News and Views |

    The envelope glycoprotein spike, the sole antigen on the Lassa virus (LASV) surface, constitutes the focal point of the host neutralizing immune response. A high-resolution structure of the trimeric LASV glycoprotein in an antibody-bound form illuminates the molecular architecture of the antigen and reveals the mode of action of the most abundant known class of Lassa-specific human neutralizing antibodies.

    • Antra Zeltina
    •  & Thomas A Bowden
  • News and Views |

    Notch signaling is an essential cell–cell communication pathway that influences numerous cell fate decisions during development. Structural and biochemical studies of a Notch–Jagged complex dramatically advance current understanding of ligand recognition, and reveal evidence of catch-bond behavior in the complex.

    • Stephen C Blacklow
  • News and Views |

    Enzymology and structural and functional characterization of some FAD-dependent monooxygenases provide insights into degradation of tetracycline antibiotics, but also show unexpected features of substrate recognition, reaction mechanism, and competitive inhibition.

    • Sonja Petkovic
    •  & Winfried Hinrichs