Featured
-
-
Article |
Streamlined and sensitive mono- and di-ribosome profiling in yeast and human cells
A comprehensive redevelopment of the ribosome profiling workflow involves improved nuclease treatment and sequencing library preparation, enabling richer and more accurate translatome profiling with lower input and fewer technical hurdles.
- Lucas Ferguson
- , Heather E. Upton
- & Nicholas T. Ingolia
-
Article |
Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
This work describes nascent Ribo-Seq, which measures the speed of ribosome loading and polysome assembly on endogenous mRNA through a combination of 4-thiouridine labeling and ribosome footprint sequencing.
- Johanna Schott
- , Sonja Reitter
- & Georg Stoecklin
-
-
Research Highlight |
Protein translation inside synthetic membraneless organelles
Phase separation in combination with spatial targeting allows for selective translation of mRNA within synthetic membraneless organelles.
- Lei Tang
-
-
Methods in Brief |
A human tRNA atlas
-
Tools in Brief |
Building a better TRAP for translation
-
Tools in Brief |
RiboHMM finds translated sequences in ribosome footprints
-
Research Highlights |
Watching translation of single mRNAs in cells
Four studies describe methods to image the dynamics of translation at the single-transcript level in real time in living cells, with exciting results.
- Rita Strack
-
Article |
Reprogramming eukaryotic translation with ligand-responsive synthetic RNA switches
Ligand-triggered ribosomal frameshifting allows control of the relative stoichiometry of two proteins and enables the building of logic gates from a single mRNA.
- Andrew V Anzalone
- , Annie J Lin
- & Virginia W Cornish
-
Methods in Brief |
Mapping active translation of single mRNAs
-
Methods in Brief |
Multiplexed single-cell protein and RNA measurements
-
Brief Communication |
Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale
This report describes an imaging and analysis platform enabling high-throughput single-molecule fluorescence investigations of fast, transient molecular recognition events.
- Manuel F Juette
- , Daniel S Terry
- & Scott C Blanchard
-
Research Highlights |
Watching proteins fold on the ribosome
Researchers monitor cotranslational protein folding in real time.
- Rita Strack
-
Article |
Detecting actively translated open reading frames in ribosome profiling data
RiboTaper quantifies the three-nucleotide periodicity in Ribo-seq data to find translated open reading frames (ORFs). The de novo inferred set of ORFs comprehensively defines the cellular proteome across a wide expression range and comprises few additional translated noncoding regions.
- Lorenzo Calviello
- , Neelanjan Mukherjee
- & Uwe Ohler
-
Research Highlights |
Inseparable ribosomes
Fusing ribosomal subunits provides answers to basic questions about their function and opens the door to exciting engineering possibilities.
- Nicole Rusk
-
Research Highlights |
RNA catch and release
The binding of single RNA molecules to individual proteins can be observed in the subcellular compartments of living cells.
- Stéphane Larochelle
-
Methods in Brief |
A TRICK for studying translation
-
This Month |
Erin Margaret Schuman
Birth-dating proteins and shaping an institute from the first building block.
- Vivien Marx
-
Research Highlights |
A global look at local translation
Proximity-specific ribosome profiling reveals the exquisite specificity of translation at the endoplasmic reticulum and mitochondrial outer membrane.
- Rita Strack
-
Methods in Brief |
Quantifying protein synthesis rates, absolutely
-
Article |
Parallel measurement of dynamic changes in translation rates in single cells
A system to monitor translation regulation in living cells is reported. By fusing a fluorescent reporter that has a controllable destabilization domain to translation regulatory motifs, the authors analyze the contribution of these motifs to changes in translation in individual cells under different experimental situations.
- Kyuho Han
- , Ariel Jaimovich
- & Tobias Meyer
-
Methods in Brief |
Allelic effects on translation
-
Brief Communication |
Genome-wide profiling of human cap-independent translation-enhancing elements
A method to identify genomic elements that promote cap-independent translation is described and is used to identify such elements in the human genome.
- Brian P Wellensiek
- , Andrew C Larsen
- & John C Chaput
-
Article |
Precise and reliable gene expression via standard transcription and translation initiation elements
By using a bicistronic design, with a leader peptide that overlaps with and contains the Shine-Dalgarno site for a downstream gene of interest, the authors demonstrate reliable, context-independent gene expression.
- Vivek K Mutalik
- , Joao C Guimaraes
- & Drew Endy
-
Article |
Quantitative estimation of activity and quality for collections of functional genetic elements
This linear ANOVA-based method quantifies the activity of different combinations of genetic elements and assigns a score that indicates the variation in performance across changing contexts.
- Vivek K Mutalik
- , Joao C Guimaraes
- & Adam P Arkin
-
Research Highlights |
Ribosomes, start your engines
Tracking single molecules in real time reveals the hidden dynamics of translational initiation.
- Tal Nawy
-
Tools in Brief |
Watching protein synthesis
-
Methods in Brief |
Ribosome profiling in bacteria
-
Research Highlights |
Better living through biochemistry
In-depth mass spectrometric analysis reveals how cells survive stress by coordinating various enzymes that modify RNAs involved in protein synthesis.
- Michael Eisenstein
-
Research Highlights |
The birth of a ribosome
A team of researchers applied a 'discovery single-particle profiling' experimental strategy to visualize the assembly of the ribosome via time-resolved electron microscopy.
- Allison Doerr
-
Research Highlights |
Made locally
Visualization of newly synthesized proteomes using conventional fluorescence microscopy reveals the local nature of protein translation.
- Erika Pastrana
-
Research Highlights |
A glow on protein synthesis
Applying technology developed for next-generation DNA sequencing to study translation, researchers watch individual ribosomes string together amino acids in real time.
- Irene Kaganman
-
Research Highlights |
Encoding the unnatural
Researchers evolved a ribosome that efficiently decodes quadruplet codons, thus opening up the possibility of genetically incorporating multiple unnatural amino acids into proteins.
- Allison Doerr
Learning structural heterogeneity from cryo-electron sub-tomograms with tomoDRGN