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This protocol describes how to obtain 100- to 400-μm-thick slices of a living myocardium from rodents, pigs, humans and dogs that retain the native multicellularity, architecture and physiology of the heart.
This protocol describes how to establish explant cultures of intact zebrafish hearts. The explants can be used to study the regeneration of cardiac tissues such as the epicardium, can be maintained for up to 30 d and are amenable to live imaging.
This protocol describes a strategy for generating 3D prostate organoid cultures from healthy mouse and human prostate cells (either bulk or FAC-sorted single luminal and basal cells), metastatic prostate cancer lesions and circulating tumor cells.