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In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.
A DNA-based reaction network has now been developed that creates a French flag pattern with immobile and sharp borders from a shallow initial concentration gradient. The output pattern can be used to control the macroscopic organization of DNA-decorated particles thereby inducing a French flag pattern of colloidal aggregation.
DNA tethers guide the self-assembly of colloidal metal nanoparticles into three-dimensional optical metamaterials. The observation of epsilon-near-zero behaviour in nanoparticle-based materials indicates that bottom-up assembly may be a viable solution to current challenges in the manufacture of metamaterials.