Featured
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Letter |
Genome editing retraces the evolution of toxin resistance in the monarch butterfly
CRISPR–Cas9 engineering of the Drosophila Atpα gene (encoding the α-subunit of the sodium pump) is used to study the ability of mutations that evolved independently in several insect orders to confer resistance to keystone plant toxins.
- Marianthi Karageorgi
- , Simon C. Groen
- & Noah K. Whiteman
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Article |
Identification of essential genes for cancer immunotherapy
The authors describe a two-cell-type CRISPR screen to identify tumour-intrinsic genes that regulate the sensitivity of cancer cells to effector T cell function.
- Shashank J. Patel
- , Neville E. Sanjana
- & Nicholas P. Restifo
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Article |
High-throughput discovery of novel developmental phenotypes
Identification and characterization, using a comprehensive embryonic phenotyping pipeline, of 410 lethal alleles during the generation of the first 1,751 of 5,000 unique gene knockouts produced by the International Mouse Phenotyping Consortium.
- Mary E. Dickinson
- , Ann M. Flenniken
- & Stephen A. Murray
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Letter |
High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells
This study describes the construction of a focused CRISPR/Cas-based lentiviral library in human cells and a method of gene identification based on functional screening and high-throughput sequencing analysis.
- Yuexin Zhou
- , Shiyou Zhu
- & Wensheng Wei
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Research Highlights |
A way to save sickle cells
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Article |
A conditional knockout resource for the genome-wide study of mouse gene function
- William C. Skarnes
- , Barry Rosen
- & Allan Bradley
A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol