Mutagenesis

Definition

Mutagenesis is the process of generating a genetic mutation. This may occur spontaneously or be induced by mutagens. Researchers also use a number of techniques to create mutations, including transposon mutagenesis to generate random gene knockouts, and site-directed mutagenesis, which utilises the polymerase chain reaction to introduce specific mutations.

Latest Research and Reviews

  • Research |

    Jos Jonkers, Lodewyk Wessels and colleagues use a Sleeping Beauty transposon mutagenesis screen to identify genes required for invasive lobular breast carcinoma formation (ILC) in mice. They find recurrent and mutually exclusive insertions in Myh9, Ppp1r12a, Ppp1r12b and Trp53bp2, which are implicated in the actin cytoskeleton regulation pathway and have been found to be altered in human ILC breast cancer.

    • Sjors M Kas
    • , Julian R de Ruiter
    • , Koen Schipper
    • , Stefano Annunziato
    • , Eva Schut
    • , Sjoerd Klarenbeek
    • , Anne Paulien Drenth
    • , Eline van der Burg
    • , Christiaan Klijn
    • , Jelle J ten Hoeve
    • , David J Adams
    • , Marco J Koudijs
    • , Jelle Wesseling
    • , Micha Nethe
    • , Lodewyk F A Wessels
    •  & Jos Jonkers
    Nature Genetics 49, 1219–1230
  • Research |

    Alex Kentsis and colleagues identify somatic genomic rearrangements in primary human rhabdoid tumors characterized by deletions and inversions involving PGBD5-specific signal sequences at their breakpoints. They further show that ectopic expression of PGBD5 in primary immortalized human cells is sufficient to promote cell transformation in vitro and in immunodeficient mice in vivo, thus defining PGBD5 as an oncogenic mutator and providing a plausible mechanism for site-specific DNA rearrangements in solid tumors.

    • Anton G Henssen
    • , Richard Koche
    • , Jiali Zhuang
    • , Eileen Jiang
    • , Casie Reed
    • , Amy Eisenberg
    • , Eric Still
    • , Ian C MacArthur
    • , Elias Rodríguez-Fos
    • , Santiago Gonzalez
    • , Montserrat Puiggròs
    • , Andrew N Blackford
    • , Christopher E Mason
    • , Elisa de Stanchina
    • , Mithat Gönen
    • , Anne-Katrin Emde
    • , Minita Shah
    • , Kanika Arora
    • , Catherine Reeves
    • , Nicholas D Socci
    • , Elizabeth Perlman
    • , Cristina R Antonescu
    • , Charles W M Roberts
    • , Hanno Steen
    • , Elizabeth Mullen
    • , Stephen P Jackson
    • , David Torrents
    • , Zhiping Weng
    • , Scott A Armstrong
    •  & Alex Kentsis
    Nature Genetics 49, 1005–1014
  • Research | | open

    CRISPR-Cas9 screens are powerful high-throughput tools but can be confounded by nuclease toxicity. Here the authors design a library of variable length gRNAs with thousands of negative controls, including the targeting of ‘safe’ loci to account for on-target site DNA damage toxicity.

    • David W. Morgens
    • , Michael Wainberg
    • , Evan A. Boyle
    • , Oana Ursu
    • , Carlos L. Araya
    • , C. Kimberly Tsui
    • , Michael S. Haney
    • , Gaelen T. Hess
    • , Kyuho Han
    • , Edwin E. Jeng
    • , Amy Li
    • , Michael P. Snyder
    • , William J. Greenleaf
    • , Anshul Kundaje
    •  & Michael C. Bassik

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