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| Open AccessHigh-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry
Real-time deformability cytometry (RT-DC) is used for mechanical cell phenotyping but is limited to a single snapshot per cell and can only measure elastic modulus. Here the authors introduce dynamic RT-DC which can measure elasticity and viscosity in single cells by following dynamic cell shape changes.
- Bob Fregin
- , Fabian Czerwinski
- & Oliver Otto
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Article
| Open AccessLignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR
The interactions of lignin with polysaccharides in plant secondary cell walls are not well understood. Here the authors employ solid-state NMR measurements to analyse intact stems of maize, Arabidopsis, switchgrass and rice and observe that lignin self-aggregates and forms highly hydrophobic microdomains that make extensive surface contacts to xylan.
- Xue Kang
- , Alex Kirui
- & Tuo Wang
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Article
| Open AccessDomain insertion permissibility-guided engineering of allostery in ion channels
Allostery is a fundamental principle of protein regulation that remains challenging to engineer. Here authors screen human Inward Rectifier K + Channel Kir2.1 for permissibility to domain insertions and propose that differential permissibility is a metric of latent allosteric capacity in Kir2.1.
- Willow Coyote-Maestas
- , Yungui He
- & Daniel Schmidt
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Article
| Open AccessKinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity
Deubiquitinating enzymes (DUBs) are critical regulators of cellular processes by removing ubiquitin from specific targets. Here global kinetic modelling reveals the mechanism by which the low intrinsic activity of USP7 is substantially enhanced on a specific physiological target.
- Robbert Q. Kim
- , Paul P. Geurink
- & Titia K. Sixma
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Article
| Open AccessMechanical loading of desmosomes depends on the magnitude and orientation of external stress
Desmosomes are intercellular adhesion complexes that connect the intermediate filament cytoskeletons of neighboring cells but direct evidence for their load-bearing nature is lacking. Here the authors develop FRET-based tension sensors to measure the forces experienced by desmoplakin and infer that desmosomes become mechanically loaded when cells are exposed to external mechanical stresses.
- Andrew J. Price
- , Anna-Lena Cost
- & Carsten Grashoff
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Article
| Open AccessCompositional adaptability in NPM1-SURF6 scaffolding networks enabled by dynamic switching of phase separation mechanisms
The nucleolus is a membrane-less organelle and both Nucleophosmin (NPM1) and Surfeit locus protein 6 (SURF6) are abundant proteins within the nucleolus. Here the authors employ biophysical methods to study the properties of NPM1-S6N droplets and provide insights into the role of SURF6 in maintaining and modulating the liquid-like structure of the nucleolus.
- Mylene C. Ferrolino
- , Diana M. Mitrea
- & Richard W. Kriwacki
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Article
| Open AccessEnergy landscape underlying spontaneous insertion and folding of an alpha-helical transmembrane protein into a bilayer
The detailed folding mechanisms of membrane proteins in their natural bilayer-like environments remains poorly understood due to the lack of tools for measuring stabilities and kinetics. Here, by simulating the folding of GlpG in a bilayer, the authors provide support for the helical-hairpin hypothesis and prompt a re-evaluation of a long-standing paradigm, the two-stage hypothesis.
- Wei Lu
- , Nicholas P. Schafer
- & Peter G. Wolynes
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Article
| Open AccessStructural snapshot of a bacterial phytochrome in its functional intermediate state
Phytochromes are photoreceptors that are present in plants, bacteria and fungi. Here the authors present crystal structures of the phytochrome Agp2 from Agrobacterium fabrum in the parent Pfr state as well as a functional Meta-F intermediate and discuss mechanistic implications for photoconversion.
- Andrea Schmidt
- , Luisa Sauthof
- & Patrick Scheerer
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Article
| Open AccessTwo-step self-assembly of a spider silk molecular clamp
Molecular details that underlie mechanical properties of spider silk are of great interest to material scientists. Here, the authors report a previously unknown three-state mechanism of folding and an expanded structure of a spider silk protein that may contribute to elasticity of spider silk.
- Charlotte Rat
- , Julia C. Heiby
- & Hannes Neuweiler
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Article
| Open AccessCalcium stabilizes the strongest protein fold
Staphylococcal pathogens adhere to their human targets using adhesins, which can withstand extremely high forces. Here, authors use single-molecule force spectroscopy to determine the similarly high unfolding forces of B domains that link the adhesin to the bacterium.
- Lukas F. Milles
- , Eduard M. Unterauer
- & Hermann E. Gaub
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Article
| Open AccessTransition path times of coupled folding and binding reveal the formation of an encounter complex
How interactions between binding partners form or break is hidden in the transition paths from the encounter to the formation of a stable complex. Here authors use single‐molecule spectroscopy to measure the transition path times for the association of two intrinsically disordered proteins that form a folded dimer upon binding and identify a metastable encounter complex.
- Flurin Sturzenegger
- , Franziska Zosel
- & Benjamin Schuler
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Article
| Open AccessDynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
Metal ions at the active site of an enzyme act as cofactors and their dynamic fluctuations might influence enzyme activity. Here authors use single-molecule FRET to study λ-exonuclease and find that metal-ion-coordination is correlated with enzymatic reaction-steps.
- Wonseok Hwang
- , Jungmin Yoo
- & Gwangrog Lee
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Article
| Open AccessA guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases
How G-quadruplexes (G4s) are resolved by helicases is still a matter of investigation. Here the authors provide mechanistic insight into G4s unwinding by presenting a crystal structure of resolved G4 DNA and the G4 binding domain of RecQ helicase from the bacterium Cronobacter sakazakii.
- Andrew F. Voter
- , Yupeng Qiu
- & James L. Keck
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Article
| Open AccessIntracellular nucleosomes constrain a DNA linking number difference of −1.26 that reconciles the Lk paradox
There had been an enduring discrepancy between theoretical and observed measurement of the DNA linking number (∆Lk) constrained by nucleosomes. Here the authors provide measurements of the ∆Lk constrained by individual nucleosomes in native chromatin that reconcile this discrepancy.
- Joana Segura
- , Ricky S. Joshi
- & Joaquim Roca
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Article
| Open AccessThe MinDE system is a generic spatial cue for membrane protein distribution in vitro
The E. coli MinCDE system oscillates between cell poles to position the main division protein FtsZ. Here authors use in vitro reconstitution to show that MinDE oscillations also regulate unrelated membrane proteins spatiotemporally into patterns and gradients by forming a moving physical barrier.
- Beatrice Ramm
- , Philipp Glock
- & Petra Schwille
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Article
| Open AccessThe evolution of multiple active site configurations in a designed enzyme
Generation and iterative optimization of designed enzymes can provide valuable insights for a more efficient catalysis. Here the authors have followed the iterative improvement of a designed Kemp eliminase and show that remote point mutations could remodel the designed active site via substantial conformational reorganization.
- Nan-Sook Hong
- , Dušan Petrović
- & Colin J. Jackson
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Article
| Open AccessIdentifying weak interdomain interactions that stabilize the supertertiary structure of the N-terminal tandem PDZ domains of PSD-95
Biologically relevant weak and transient interdomain interactions within proteins are difficult to analyze. Here, the authors combine multiscale molecular dynamics simulations and high-precision FRET experiments to characterize interactions between the tandem PDZ domains of PSD-95, revealing previously hidden conformational states.
- Inna S. Yanez Orozco
- , Frank A. Mindlin
- & Hugo Sanabria
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Article
| Open AccessFocused clamping of a single neuronal SNARE complex by complexin under high mechanical tension
The SNARE complex enables the fusion of synaptic vesicles with presynaptic membrane via a zippering process that is modulated by the protein complexin, though the precise mechanism remains unclear. Here, the authors used magnetic tweezers to show how complexin prepares a SNARE complex for fusion under mechanical tension.
- Min Ju Shon
- , Haesoo Kim
- & Tae-Young Yoon
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Article
| Open AccessHIF-2α-pVHL complex reveals broad genotype-phenotype correlations in HIF-2α-driven disease
Hypoxia inducible factor (HIF)-2α transcription factor is mutated in polycythemia and various neuroendocrine tumors. Here the authors present the crystal structure of a HIF-2α peptide bound to the pVHL-elongin B-elongin C (VBC) heterotrimeric complex and propose a classification scheme for HIF-2α mutations that helps to predict disease phenotype outcome.
- Daniel Tarade
- , Claire M. Robinson
- & Michael Ohh
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Article
| Open AccessA proline switch explains kinetic heterogeneity in a coupled folding and binding reaction
How intrinsically disordered proteins (IDPs) undergo a coupled folding and binding reaction with their molecular targets remains to be understood. Here authors use single-molecule FRET to assess the contribution of cis/trans isomerization of peptidyl-prolyl bonds in regulating IDP interactions.
- Franziska Zosel
- , Davide Mercadante
- & Benjamin Schuler
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Article
| Open AccessMapping the energy landscapes of supramolecular assembly by thermal hysteresis
Complex assembly pathways often involve transient, partly-formed intermediates that are challenging to characterize. Here, the authors present a simple and rapid spectroscopic thermal hysteresis method for mapping the energy landscapes of supramolecular assembly.
- Robert W. Harkness V
- , Nicole Avakyan
- & Anthony K. Mittermaier
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Article
| Open AccessInterphase human chromosome exhibits out of equilibrium glassy dynamics
The 3D organization of chromosomes within the nuclear space is important for biological functions. Here the authors model chromosomes as self-avoiding copolymers with distinct epigenetic states, and show that the features of experimentally generated contact maps can be reproduced and their dynamics predicted.
- Guang Shi
- , Lei Liu
- & D. Thirumalai
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Article
| Open AccessThe solute carrier SLC9C1 is a Na+/H+-exchanger gated by an S4-type voltage-sensor and cyclic-nucleotide binding
The sperm-specific solute carrier SLC9C1 is a phylogenetic chimaera that carries a voltage-sensing (VSD) and a cyclic nucleotide-binding domain (CNBD). Here authors show by electrophysiology and fluorimetry that SLC9C1 is a genuine Na+/H+ exchanger gated by voltage and cAMP.
- F. Windler
- , W. Bönigk
- & U. B. Kaupp
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Article
| Open AccessStructural basis for endotoxin neutralisation and anti-inflammatory activity of thrombin-derived C-terminal peptides
Thrombin-derived C-terminal peptides (TCPs) have anti-endotoxic functions in wounds by binding to bacterial lipopolysaccharide (LPS) and Gram-negative bacteria. Here authors use a spectrum of biophysical techniques to determine the conformation of a TCP in complex with LPS and define the interaction between TCPs and CD14.
- Rathi Saravanan
- , Daniel A Holdbrook
- & Artur Schmidtchen
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Article
| Open AccessSingle-molecule insights into surface-mediated homochirality in hierarchical peptide assembly
Most chiral molecules and structures in living organisms exist as single enantiomers, but why? Here, the authors investigated surface-mediated homochirality on the single-molecule level and show that it can be triggered by the chirality unbalance of two adsorption configuration monomers.
- Yumin Chen
- , Ke Deng
- & Chen Wang
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Article
| Open AccessHydrophobic pore gates regulate ion permeation in polycystic kidney disease 2 and 2L1 channels
Mutations in the cation channel PKD2 cause human autosomal dominant polycystic kidney disease but its channel function and gating mechanism are poorly understood. Here authors study PKD2 using electrophysiology and cryo-EM, which identifies hydrophobic gates and proposes a gating mechanism for PKD2.
- Wang Zheng
- , Xiaoyong Yang
- & Xing-Zhen Chen
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Article
| Open AccessBudding-like division of all-aqueous emulsion droplets modulated by networks of protein nanofibrils
The cytoskeleton, a network of fibrils, controls how cells divide. Here, the authors show that synthetic protein fibrils added to an emulsion can control the division of droplets and that this method can be used to control the morphology of microparticles during biomaterial preparation.
- Yang Song
- , Thomas C. T. Michaels
- & Ho Cheung Shum
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Article
| Open AccessRhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 Å structure of the adenylyl cyclase domain
Cyclic AMP and cGMP orchestrate a variety of cellular responses. Here, authors characterize the cGMP producing rhodopsin-guanylyl cyclase from C. anguillulae and derived adenylyl cyclase by a biochemical and structural approach which demonstrates the usability of these cyclases for optogenetic applications.
- Ulrike Scheib
- , Matthias Broser
- & Peter Hegemann
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Article
| Open AccessDefining a conformational ensemble that directs activation of PPARγ
Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor. Here the authors provide insights into PPARγ activation by combining fluorine (19F) NMR and molecular dynamics simulations to characterize the nuclear receptor conformational ensemble in solution and the response of this ensemble to ligand and coregulatory peptide binding.
- Ian M. Chrisman
- , Michelle D. Nemetchek
- & Travis S. Hughes
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Article
| Open AccessSubstrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site
Sialic acid transporters (SiaT) are required for sialic acid uptake in a number of human pathogens and are of interest as targets for antimicrobial drug development. Here the authors present the substrate bound SiaT structure from the uropathogen Proteus mirabilis and provide insights into the mechanism of sialic acid transport.
- Weixiao Y. Wahlgren
- , Elin Dunevall
- & Rosmarie Friemann
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Article
| Open AccessDynamic tuneable G protein-coupled receptor monomer-dimer populations
Evidence suggests oligomerisation of G protein-coupled receptors in membranes, but this is controversial. Here, authors use single-molecule and ensemble FRET, and spectroscopy to show that the neurotensin receptor 1 forms multiple dimer conformations that interconvert - “rolling” interfaces.
- Patricia M. Dijkman
- , Oliver K. Castell
- & Anthony Watts
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Article
| Open AccessThe cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation
Mutations in superoxide dismutase-1 (SOD1) cause amyotrophic lateral sclerosis (ALS). Here the authors present the SOD1 crystal structure bound to the small cysteine-reactive molecule ebselen and show that ebselen is a chaperone for SOD1.
- Michael J. Capper
- , Gareth S. A. Wright
- & S. Samar Hasnain
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Article
| Open AccessCapping protein-controlled actin polymerization shapes lipid membranes
Cell membrane protrusions and invaginations are both driven by actin assembly but the mechanism leading to different membrane shapes is unknown. Using a minimal system and modelling the authors reconstitute the deformation modes and identify capping protein as a regulator of both deformation types.
- Katharina Dürre
- , Felix C. Keber
- & Andreas R. Bausch
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Article
| Open AccessRescue of conformational dynamics in enzyme catalysis by directed evolution
A key challenge in the field of protein design and evolution is to understand the mechanisms by which directed evolution is improving enzymes. Here the authors combine different biophysical methods and give mechanistic insights into how directed evolution increases the catalytic efficiency of human peptidyl-prolyl cis/trans isomerase CypA.
- Renee Otten
- , Lin Liu
- & James S. Fraser
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Article
| Open AccessRodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape
Membrane protein RodZ interacts with the actin-like protein MreB, which coordinates cell-wall insertion to maintain the typical rod-like shape of E. coli cells. Here, the authors provide evidence that RodZ modulates the biophysical properties of MreB and alters the spatial organization of cell-wall growth.
- Alexandre Colavin
- , Handuo Shi
- & Kerwyn Casey Huang
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Article
| Open AccessBiomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironment
The efficacy of stimuli-responsive enzyme delivery systems is usually limited to in vitro applications. Here the authors form artificial organelles by inserting stimuli-responsive protein gates in membranes of polymersomes loaded with enzymes and obtain a triggered functionality both in vitro and in vivo.
- T. Einfalt
- , D. Witzigmann
- & C. G. Palivan
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Article
| Open AccessLarge enhancement of response times of a protein conformational switch by computational design
The rational optimization of response times of protein conformational switches is a major challenge for biomolecular switch design. Here the authors present a generally applicable computational design strategy that in combination with biophysical experiments can improve response times using a Ca2+-sensor as an example.
- Alex J. DeGrave
- , Jeung-Hoi Ha
- & Lillian T. Chong
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Article
| Open AccessSelf-interaction of NPM1 modulates multiple mechanisms of liquid–liquid phase separation
The nucleolus is a membrane-less organelle formed through liquid–liquid phase separation (LLPS). Here the authors use biophysical methods and show that the nucleolar protein nucleophosmin (NPM1) also undergoes LLPS through homotypic, inter-NPM1 interactions and discuss implications for the ribosome biogenesis process.
- Diana M. Mitrea
- , Jaclyn A. Cika
- & Richard W. Kriwacki
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Article
| Open AccessInduction of long-lived room temperature phosphorescence of carbon dots by water in hydrogen-bonded matrices
A long phosphorescence lifetime is desirable for imaging and detection, however, phosphorescence is often quenched in aqueous environments limiting applications. Here, Li et al. present a strategy for the long-lived phosphorescence of carbon dots in water due to multiple hydrogen-bonding interactions
- Qijun Li
- , Ming Zhou
- & Jing Shi
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Article
| Open AccessElectric fields control the orientation of peptides irreversibly immobilized on radical-functionalized surfaces
Implanted materials can be rejected by the body, and coating the surfaces with peptides is seen as an option to overcome this problem. Here, the authors investigated how pH and electric fields can be used to prepare defined peptide coatings.
- Lewis J. Martin
- , Behnam Akhavan
- & Marcela M. M. Bilek
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Article
| Open AccessEpsin and Sla2 form assemblies through phospholipid interfaces
Adapter proteins assist clathrin coated pit assembly. Here, the authors combine native mass spectrometry, crystallography and SAXS measurements and show that the membrane–proximal domains of the adaptor proteins epsin and Sla2 form complexes mediated through phosphatidylinositol 4,5-bisphosphate interfaces leading to assembly formation.
- Maria M. Garcia-Alai
- , Johannes Heidemann
- & Rob Meijers
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Article
| Open AccessSingle-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α
Chromatin fibers undergo continuous structural rearrangements but their dynamic architecture is poorly understood. Here, the authors use single-molecule FRET to determine the structural states and interconversion kinetics of chromatin fibers, monitoring their effector protein-dependent dynamic motions.
- Sinan Kilic
- , Suren Felekyan
- & Beat Fierz
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Article
| Open AccessProtein conformational flexibility modulates kinetics and thermodynamics of drug binding
An understanding of the dynamics of drug binding and unbinding processes is important for drug discovery. Here, the authors give insights into the binding mechanism of small drug-like molecules to human Hsp90 by combining thermodynamics and kinetics studies as well as molecular dynamics simulations.
- M. Amaral
- , D. B. Kokh
- & M. Frech
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Article
| Open AccessSurvey of solution dynamics in Src kinase reveals allosteric cross talk between the ligand binding and regulatory sites
Src is a prototypical signaling non-receptor protein tyrosine kinase that interconverts between distinct conformations. Here the authors use variants of the kinase-inhibitor dasatinib to define three specific conformational states of the Src kinase and shed insight on the effect of conformation-specific inhibitors on Src dynamics.
- Michael Tong
- , Jeff G. Pelton
- & Markus A. Seeliger
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Article
| Open AccessMechanistic insight into TRIP13-catalyzed Mad2 structural transition and spindle checkpoint silencing
The spindle checkpoint ensures the fidelity of chromosome segregation during mitosis and meiosis. Here the authors use a combination of biochemical and structural biology approaches to show how the TRIP13 ATPase and its adaptor, p31comet, catalyze the conversion of the checkpoint protein Mad2 between latent and active forms
- Melissa L. Brulotte
- , Byung-Cheon Jeong
- & Xuelian Luo
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Article
| Open AccessThe interdomain flexible linker of the polypeptide GalNAc transferases dictates their long-range glycosylation preferences
GalNAc transferases’ (GalNAc-Ts) catalytic domains are connected to a lectin domain through a flexible linker. Here the authors present a structural analysis of GalNAc-T4 that implicates the linker region as modulator of the orientations of the lectin domain, which in turn imparts substrate specificity.
- Matilde de las Rivas
- , Erandi Lira-Navarrete
- & Ramon Hurtado-Guerrero
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Article
| Open AccessNDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription
Myosin VI (MVI) is known to interact with RNA Polymerase II and to play non-cytoplasmic roles in cells. Here, the authors provide evidence that the transcription co-activator NDP52 regulates MVI binding to DNA and that MVI interacts with nuclear receptors to drive gene expression.
- Natalia Fili
- , Yukti Hari-Gupta
- & Christopher P. Toseland
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Article
| Open AccessInactivation of KCNQ1 potassium channels reveals dynamic coupling between voltage sensing and pore opening
KCNQ1 is a voltage-gated potassium channel that is important in cardiac and epithelial function. Here the authors present a mechanism for KCNQ1 activation and inactivation in which voltage sensor activation promotes pore opening more effectively in the intermediate open state than the fully open state, generating inactivation.
- Panpan Hou
- , Jodene Eldstrom
- & Jianmin Cui
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Article
| Open AccessSingle-molecule visualization of conformational changes and substrate transport in the vitamin B12 ABC importer BtuCD-F
The vitamin B12 importer BtuCD-F is a type II ATP-binding cassette transporter. Here the authors use single-molecule fluorescence techniques to analyse ATP hydrolysis and substrate transport in individual BtuCD-F complexes embedded in liposomes, and propose a transport model.
- Joris M. H. Goudsmits
- , Dirk Jan Slotboom
- & Antoine M. van Oijen