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Open Access
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Article |
Anti-TIGIT antibody improves PD-L1 blockade through myeloid and Treg cells
A high baseline of intratumoural macrophages and regulatory T cells is associated with better outcomes in patients with non-small cell lung cancer treated with atezolizumab plus tiragolumab, but not with atezolizumab alone.
- Xiangnan Guan
- , Ruozhen Hu
- & Namrata S. Patil
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Article
| Open AccessAn atlas of epithelial cell states and plasticity in lung adenocarcinoma
Analyses of single epithelial cells from early-stage lung adenocarcinoma and normal lung identifies a population of intermediate cells that may have an increased likelihood of transforming to tumour cells after injury such as tobacco exposure.
- Guangchun Han
- , Ansam Sinjab
- & Humam Kadara
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Article |
p53 governs an AT1 differentiation programme in lung cancer suppression
p53 is shown to have a pivotal role in the differentiation of alveolar type 1 cells in cancer and alveolar repair after injury, and loss of this governance can promote diseases such as lung adenocarcinoma.
- Alyssa M. Kaiser
- , Alberto Gatto
- & Laura D. Attardi
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Article |
KRAS(G12D) drives lepidic adenocarcinoma through stem-cell reprogramming
A study identifies the AT1 cell as a cell of origin for lung adenocarcinoma, and demonstrates that expression of oncogenic KRAS in differentiated AT1 cells reprograms them back into AT2 stem cells that generate indolent lepidic tumours.
- Nicholas H. Juul
- , Jung-Ki Yoon
- & Tushar J. Desai
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Article |
Therapy-induced APOBEC3A drives evolution of persistent cancer cells
Induction of APOBEC3A in response to targeted therapies drives evolution of drug-tolerant persister cells, suggesting that its suppression may represent a potential therapeutic strategy in the prevention of acquired resistance to lung cancer targeted therapy.
- Hideko Isozaki
- , Ramin Sakhtemani
- & Aaron N. Hata
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Article |
Tracking early lung cancer metastatic dissemination in TRACERx using ctDNA
Measurements of subclonal expansion of ctDNA in the plasma before surgery may enable the prediction of future metastatic subclones, offering the possibility for early intervention in patients with non-small-cell lung cancer.
- Christopher Abbosh
- , Alexander M. Frankell
- & Charles Swanton
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Article
| Open AccessGenomic–transcriptomic evolution in lung cancer and metastasis
Computational and machine-learning approaches that integrate genomic and transcriptomic variation from paired primary and metastatic non-small cell lung cancer samples from the TRACERx cohort reveal the role of transcriptional events in tumour evolution.
- Carlos Martínez-Ruiz
- , James R. M. Black
- & Nicholas McGranahan
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Article
| Open AccessThe evolution of non-small cell lung cancer metastases in TRACERx
A longitudinal evolutionary analysis of 126 lung cancer patients with metastatic disease reveals the timing of metastatic divergence, modes of dissemination and the genomic events subject to selection during the metastatic transition.
- Maise Al Bakir
- , Ariana Huebner
- & Charles Swanton
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Article
| Open AccessAntibodies against endogenous retroviruses promote lung cancer immunotherapy
In lung adenocarcinoma, antibodies against endogenous retroviruses promote anti-tumour activity, and expression of endogenous retroviruses can predict outcomes of immunotherapy.
- Kevin W. Ng
- , Jesse Boumelha
- & George Kassiotis
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Article |
Lung adenocarcinoma promotion by air pollutants
Combination of epidemiology, preclinical models and ultradeep DNA profiling of clinical cohorts unpicks the inflammatory mechanism by which air pollution promotes lung cancer
- William Hill
- , Emilia L. Lim
- & Charles Swanton
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Article
| Open AccessSpatial mapping of mitochondrial networks and bioenergetics in lung cancer
A study describing an approach that combines imaging and profiling techniques to structurally and functionally analyse lung cancer in vivo, revealing heterogeneous mitochondrial networks and an association between bioenergetic phenotypes and mitochondrial organization and function.
- Mingqi Han
- , Eric A. Bushong
- & David B. Shackelford
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Article
| Open AccessSingle-cell spatial landscapes of the lung tumour immune microenvironment
Using imaging mass cytometry, the tumour and immunological spatial landscapes of 416 lung adenocarcinomas are characterized, which, when combined with deep learning, can predict clinical outcomes with high accuracy.
- Mark Sorin
- , Morteza Rezanejad
- & Logan A. Walsh
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Article |
Deciphering the immunopeptidome in vivo reveals new tumour antigens
A newly developed genetically engineered mouse model enables the analysis of specific antigen presentation in vivo, providing insights into the tumour immunopeptidome and cancer progression.
- Alex M. Jaeger
- , Lauren E. Stopfer
- & Tyler Jacks
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Article |
The CLIP1–LTK fusion is an oncogenic driver in non‐small‐cell lung cancer
Whole-transcriptome sequencing of a subset of 75 non-small-cell lung cancer specimens in a multi-institutional genome screening study identified a fusion of the CLIP1 and LTK genes with transformational potential due to constitutive LTK kinase activity.
- Hiroki Izumi
- , Shingo Matsumoto
- & Koichi Goto
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Article |
Diverse alterations associated with resistance to KRAS(G12C) inhibition
Multiple treatment-emergent alterations appear in patients with advanced-stage cancer who were treated with a KRAS inhibitor.
- Yulei Zhao
- , Yonina R. Murciano-Goroff
- & Piro Lito
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Article
| Open AccessStructure-based classification predicts drug response in EGFR-mutant NSCLC
Structural classification of mutations in the epidermal growth factor receptor causing non-small cell lung cancer is a better predictor of patient outcomes following drug treatment than traditional exon-based classification.
- Jacqulyne P. Robichaux
- , Xiuning Le
- & John V. Heymach
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Article |
Elevated NSD3 histone methylation activity drives squamous cell lung cancer
The histone H3K36 methyltransferase NSD3, which is associated with the common 8p11–12 chromosomal amplification, is an oncogenic driver in lung squamous cell carcinoma.
- Gang Yuan
- , Natasha M. Flores
- & Or Gozani
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Article |
The National Lung Matrix Trial of personalized therapy in lung cancer
Current outcomes are reported from the ongoing National Lung Matrix Trial, an umbrella trial for the treatment of non-small-cell lung cancer in which patients are triaged according to their tumour genotype and matched with targeted therapeutic agents.
- Gary Middleton
- , Peter Fletcher
- & Lucinda Billingham
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Matters Arising |
Reply to: In vivo quantification of mitochondrial membrane potential
- Milica Momcilovic
- , Orian Shirihai
- & David B. Shackelford
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Article |
Integrating genomic features for non-invasive early lung cancer detection
Circulating tumour DNA in blood is analysed to identify genomic features that distinguish early-stage lung cancer patients from risk-matched controls, and these are integrated into a machine-learning method for blood-based lung cancer screening.
- Jacob J. Chabon
- , Emily G. Hamilton
- & Maximilian Diehn
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Article |
Rapid non-uniform adaptation to conformation-specific KRAS(G12C) inhibition
Populations of KRAS(G12C)-mutant cancer cells can rapidly bypass the effects of treatment with KRAS(G12C) inhibitors because a subset of cells escapes drug-induced quiescence by producing new KRAS(G12C) that is maintained in its active, drug-insensitive state.
- Jenny Y. Xue
- , Yulei Zhao
- & Piro Lito
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Article |
In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer
A positron emission tomography imaging tracer is developed to image mitochondrial function in vivo, and application of this tracer to a mouse model of lung cancer identifies distinct functional mitochondrial heterogeneity between tumour cells.
- Milica Momcilovic
- , Anthony Jones
- & David B. Shackelford
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Letter |
RB constrains lineage fidelity and multiple stages of tumour progression and metastasis
Loss of RB promotes both malignant progression and the development of metastatic disease; however, whereas reactivation of the RB pathway can revert metastatic tumour cell states to non-metastatic cell states, malignant cell proliferation is supported by MAPK–CDK2-dependent suppression of RB.
- David M. Walter
- , Travis J. Yates
- & David M. Feldser
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Article |
Neoantigen-directed immune escape in lung cancer evolution
RNA sequencing data and tumour pathology observations of non-small-cell lung cancers indicate that the immune cell microenvironment exerts strong evolutionary selection pressures that shape the immune-evasion capacity of tumours.
- Rachel Rosenthal
- , Elizabeth Larose Cadieux
- & Andrew Kidd
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Review Article |
The biology and management of non-small cell lung cancer
- Roy S. Herbst
- , Daniel Morgensztern
- & Chris Boshoff
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Letter |
A Braf kinase-inactive mutant induces lung adenocarcinoma
Kinase-inactive Braf mutants can initiate the development of lung adenocarcinoma in mice; co-expression of activated Kras enhances tumour initiation and progression, and wild-type Braf is required to sustain tumorigenesis.
- Patricia Nieto
- , Chiara Ambrogio
- & David Santamaría
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Letter |
A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma
A subset of Kras and p53 mutant cancer cells acts as a Wnt-producing niche for another cancer cell subset, and porcupine inhibition disrupts Wnt secretion in this niche, thereby suppressing proliferative potential and leading to therapeutic benefit.
- Tuomas Tammela
- , Francisco J. Sanchez-Rivera
- & Tyler Jacks
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Article |
Phylogenetic ctDNA analysis depicts early-stage lung cancer evolution
Circulating tumour DNA profiling in early-stage non-small-cell lung cancer can be used to track single-nucleotide variants in plasma to predict lung cancer relapse and identify tumour subclones involved in the metastatic process.
- Christopher Abbosh
- , Nicolai J. Birkbak
- & Charles Swanton
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Letter |
XPO1-dependent nuclear export is a druggable vulnerability in KRAS-mutant lung cancer
A multi-genomic approach identifies the addiction of KRAS-mutant lung cancer cells to XPO1-dependent nuclear export, offering a new therapeutic opportunity.
- Jimi Kim
- , Elizabeth McMillan
- & Michael A. White
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Article |
A combinatorial strategy for treating KRAS-mutant lung cancer
A systematic screen identifies FGFR1 signalling reactivation as an adaptive resistance mechanism after MEK inhibition specific for KRAS tumours, which can be targeted by combined inhibition with the clinically approved drugs trametinib and ponatinib.
- Eusebio Manchado
- , Susann Weissmueller
- & Scott W. Lowe
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Letter |
Overcoming EGFR(T790M) and EGFR(C797S) resistance with mutant-selective allosteric inhibitors
An allosteric inhibitor, EAI045, is reported that is selective for certain drug-resistant EGFR mutants, but spares the wild-type receptor; combination therapy of EAI045 with EGFR-dimerization-blocking antibodies is effective in mouse models of lung cancer driven by mutant versions of EGFR that are resistant to all previously developed inhibitors.
- Yong Jia
- , Cai-Hong Yun
- & Michael J. Eck
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Letter |
Nucleotide excision repair is impaired by binding of transcription factors to DNA
An analysis of cancer genomic data reveals an increased rate of somatic mutations at active transcription factor binding sites located both within promoter regions and distal from genes; the increased mutation rate at these genomic regions can be explained by reduced accessibility of the protein-bound DNA to nucleotide excision repair machinery.
- Radhakrishnan Sabarinathan
- , Loris Mularoni
- & Núria López-Bigas
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Letter |
Mutant Kras copy number defines metabolic reprogramming and therapeutic susceptibilities
Mutant Kras lung tumours are not a single disease but comprise two classes of tumours with distinct metabolic profiles, prognosis and therapeutic susceptibility, which can be discriminated by their relative mutant Kras allelic content.
- Emma M. Kerr
- , Edoardo Gaude
- & Carla P. Martins
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Letter |
The mutational landscapes of genetic and chemical models of Kras-driven lung cancer
Whole-exome sequencing is used to compare the mutational landscape of adenomas from three mouse models of non-small-cell lung cancer, induced either by exposure to carcinogens or by genetic mutation of Kras; the results reveal that the two types of tumour have different mutational profiles and adopt different routes to tumour development.
- Peter M. K. Westcott
- , Kyle D. Halliwill
- & Allan Balmain
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Letter |
In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system
The CRISPR/Cas system has been used to induce the Eml4–Alk chromosomal inversion in mice, a characteristic chromosomal rearrangement seen in human non-small cell lung cancers; the mice developed lung cancer and responded to the ALK inhibitor crizotinib, which is used to treat lung cancer patients with the EML4–ALK rearrangement; this general strategy can be used to engineer other disease-associated chromosomal rearrangements in mice and potentially in other organisms.
- Danilo Maddalo
- , Eusebio Manchado
- & Andrea Ventura
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Letter |
Rapid modelling of cooperating genetic events in cancer through somatic genome editing
The CRISPR/Cas system has been used in mice for genome editing to introduce genetic alterations found in human lung tumours, and these genome modifications resulted in mouse lung tumours showing different histopathologies depending on the genes altered; the CRISPR/Cas system offers improved and faster ways to create animal models of human diseases such as cancer.
- Francisco J. Sánchez-Rivera
- , Thales Papagiannakopoulos
- & Tyler Jacks
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Outlook |
Prevention: Air of danger
Carcinogens are all around us, so scientists are broadening their ideas of environmental risk.
- Rebecca Kessler
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Letter |
HMGA2 functions as a competing endogenous RNA to promote lung cancer progression
HMGA2 promotes lung cancer progression in mice and humans; in mouse and human lung cancer cells, HMGA2 competes with mRNAs like TGFBR3 for the let-7 microRNA family, and in human non-small-cell lung cancer tissue, expression levels of HMGA2 and TGFBR3 are correlated, suggesting that HMGA2 functions both as a protein-coding gene and as a non-coding RNA.
- Madhu S. Kumar
- , Elena Armenteros-Monterroso
- & Julian Downward
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News |
Studies offer ‘panoramic view’ of lung cancer
Three genome-sequencing trials may help to revamp treatments for the world’s most deadly cancer.
- Monya Baker
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Article
| Open AccessComprehensive genomic characterization of squamous cell lung cancers
Comprehensive analyses of 178 lung squamous cell carcinomas by The Cancer Genome Atlas project show that the tumour type is characterized by complex genomic alterations, with statistically recurrent mutations in 11 genes, including TP53 in nearly all samples; a potential therapeutic target is identified in most of the samples studied.
- Peter S. Hammerman
- , Michael S. Lawrence
- & Matthew Meyerson
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News & Views |
Clinical trials unite mice and humans
Anticancer 'co-clinical' trials, in which mice carrying known mutations are treated in parallel with patients enrolled in a simultaneous clinical study, could help to improve therapeutic outcome. See Letter p.613
- Leisa Johnson
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Letter |
A murine lung cancer co-clinical trial identifies genetic modifiers of therapeutic response
In parallel with an ongoing human clinical trial, genetically engineered mouse models of lung cancer with different genetic alterations are treated with chemotherapeutic agents; the results have implications for the clinical trial.
- Zhao Chen
- , Katherine Cheng
- & Kwok-Kin Wong
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News |
Targeted treatment tested as potential cancer cure
Trial will deploy genetically targeted therapy early, rather than as last resort.
- Erika Check Hayden
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Research Highlights |
Pushing back on drug resistance
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Letter |
Suppression of lung adenocarcinoma progression by Nkx2-1
- Monte M. Winslow
- , Talya L. Dayton
- & Tyler Jacks
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Outlook |
Biomarkers: Portents of malignancy
Being able to determine an individual's chances of developing cancer will greatly improve risk management strategies and recruitment to clinical trials.
- Vicki Brower
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News |
Mutations block lung-cancer treatment
Revealing the genetic changes that let tumours escape drugs offers hope for combination therapies.
- Heidi Ledford
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Letter |
FAS and NF-κB signalling modulate dependence of lung cancers on mutant EGFR
Lung cancers with activating mutations in EGFR can be treated with EGFR inhibitors, but not all tumours respond and some develop resistance. In an RNAi screen, this study searches for modifiers of the EGFR inhibitor response. It is found that inhibition of FAS and NF-κB signalling enhances the response in vitro and in vivo. In a cohort of lung cancer patients treated with EGFR inhibitors, expression of the NF-κB inhibitor IκB is associated with a better response and longer survival, indicating that combining NF-κB pathway and EGFR inhibitors may prove clinically useful.
- Trever G. Bivona
- , Haley Hieronymus
- & Charles L. Sawyers
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Editorial |
Asbestos scandal
Irresponsible policies could cause an epidemic of malignant lung disease.