Gene delivery articles within Nature Methods

Featured

• Commentary | 30 July 2013

  The transience of transient overexpression

  Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.

  • Toby J Gibson
  • , Markus Seiler
  •  & Reiner A Veitia

• Brief Communication | 31 March 2013

  Direct transfer of whole genomes from bacteria to yeast

  A direct transfer of bacterial genomic DNA to yeast enables genome engineering in genetically intractable bacteria.

  • Bogumil J Karas
  • , Jelena Jablanovic
  •  & Yo Suzuki

• Research Highlights | 27 February 2013

  A back door to the neuron

  An electrochemical gene-delivery method enables rapid modification of gene expression in postmitotic neurons in vivo, changing their identity and connectivity pattern.

  • Erika Pastrana

• Article | 13 January 2013

  Lentiviral vector–based insertional mutagenesis identifies genes associated with liver cancer

  Replication-deficient lentiviral vectors are used as insertional mutagens for cancer gene discovery in the mouse. They are applied to identify oncogenes in hepatocellular carcinoma.

  • Marco Ranzani
  • , Daniela Cesana
  •  & Eugenio Montini

• Methods in Brief | 01 January 2013

  Site-specific gene insertion

• Research Highlights | 27 March 2012

  siRNA as a sponge

  New nanostructure delivers potent dose of short interfering RNA

  • Monya Baker

• Advertising Feature: Application Note | 28 February 2012

  Expresso^® CMV system: effortless mammalian expression cloning

  • Saurabh Sen
  • , Heather Sternard
  •  & Ronald Godiska

• Brief Communication | 04 December 2011

  Controlled gene expression in primary Lgr5 organoid cultures

  The controlled overexpression or knockdown of gene expression in primary organoid cultures of mouse endodermal epithelia is described. This should enable ex vivo studies of mammalian gene function.

  • Bon-Kyoung Koo
  • , Daniel E Stange
  •  & Hans Clevers

• Research Highlights | 29 November 2011

  Nanoelectroporation

  Precise amounts of DNA and quantum dots can be moved into cells through tiny channels.

  • Monya Baker

• Article | 23 October 2011

  A scalable pipeline for highly effective genetic modification of a malaria parasite

  Reported is a recombineering pipeline for efficient and large-scale gene modification in the mouse malarial parasite Plasmodium berghei.

  • Claudia Pfander
  • , Burcu Anar
  •  & Oliver Billker

• Article | 21 August 2011

  Site-specific integration and tailoring of cassette design for sustainable gene transfer

  Presented is an experimental analysis of the stability of transgene expression, the perturbation of endogenous expression and the perturbation of epigenetic organization upon site-directed delivery of transgenes to the CCR5 and AAVS1 loci in human cells. It provides guidelines for optimal cassette design for stable and nonperturbative gene transfer.

  • Angelo Lombardo
  • , Daniela Cesana
  •  & Luigi Naldini

• Advertising Feature: Application Note | 27 May 2011

  Cellaxess^®HT cell-based assay and transfection lab

  • Sara Aspengren
  • , Michael Tokarz
  •  & Johan Pihl

• This Month | 28 October 2010

  Rolf Zeller and Javier Lopez-Rios

  Gene cutting and pasting just got a whole lot faster.

  • Monya Baker

• Brief Communication | 17 October 2010

  Efficient CNS gene delivery by intravenous injection

  Recombinant SV40 viral vectors intravenously injected into mice pretreated with mannitol effectively deliver transgenes to adult neurons in several regions of the central nervous system.

  • Jean-Pierre Louboutin
  • , Alena A Chekmasova
  •  & David S Strayer

• Article | 10 October 2010

  Specific gene transfer to neurons, endothelial cells and hematopoietic progenitors with lentiviral vectors

  A targeting method for lentiviral vectors relying on the use of single-chain antibodies recognizing cell-surface antigens is applied to generate lentiviral vectors specific for endothelial cells, hematopoietic progenitors and neurons.

  • Brigitte Anliker
  • , Tobias Abel
  •  & Christian J Buchholz

• News & Views | 01 September 2010

  C. elegans select

  The technical toolkit for Caenorhabditis elegans expands to include experimental selection using antibiotic resistance genes.

  • Helen M Chamberlin

• Brief Communication | 22 August 2010

  An antibiotic selection marker for nematode transgenesis

  A gene conferring neomycin resistance can be used for antibiotic selection in C. elegans and C. briggsae. This will permit easy maintenance of transgenic lines and facilitate single-copy insertion of transgenes. Also in this issue, a related paper reports nematode selection using puromycin.

  • Rosina Giordano-Santini
  • , Stuart Milstein
  •  & Denis Dupuy

• Advertising Feature: Application Note | 01 June 2010

  ReGenX AAV Vector Technology: a tool for in vivo screening

  • Karen Kozarsky

• Brief Communication | 07 February 2010

  A nonviral minicircle vector for deriving human iPS cells

  Simple minicircle vectors carrying four reprogramming factors induce pluripotency in adult human adipose stem cells and in neonatal fibroblasts without integration into the genome.

  • Fangjun Jia
  • , Kitchener D Wilson
  •  & Joseph C Wu