Fluorescence resonance energy transfer articles from across Nature Portfolio

Moderately efficient TADF emitters can play the role of surprisingly good sensitizers in hyperfluorescent blue organic light-emitting diodes.

Here, using single molecule FRET, the unfolding and folding of a discontinuous two-domain protein was studied. The authors find that a dynamic, intermediate population entropically limits the rate of folding while the order of domain folding is kept in a slow-folding mutant.

The precise role of cochaperones and ATP hydrolysis in driving Hsp90’s chaperone cycle is largely unclear. Here, the authors use single-molecule FRET to show that several cochaperones are necessary to establish directionality in Hsp90’s conformational cycle.

Single-molecule FRET experiments with human A2A adenosine receptor in its apo and agonist-bound states in lipid nanodiscs provide insights into its conformational dynamics and activation.

An international blind study confirms that smFRET measurements on dynamic proteins are highly reproducible across instruments, analysis procedures and timescales, further highlighting the promise of smFRET for dynamic structural biology.

Gibberellins (GAs) control key growth and developmental processes in plants. Real-time monitoring of GA concentrations in living tissues is critical for understanding the actions of this hormone class. A first-generation optogenetic GA-nano-indicator now illuminates the effects of GA levels on cell length and light signalling.

A nanoscopic force clamp enables high-throughput single-molecule analysis of DNA under tension without connection to a macroscopic instrument.

An integrated single-molecule fluorescence approach enables the study of nano-to-millisecond protein conformational dynamics in living mammalian cells.