Featured
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Article |
Detecting chromatin interactions between and along sister chromatids with SisterC
It remains impossible using conventional Hi-C to differentiate interactions between and along sister chromatids. SisterC relies on selective destruction of nascent DNA and in combination with Hi-C offers a means to study intra- and inter-sister interactions independently.
- Marlies E. Oomen
- , Adam K. Hedger
- & Job Dekker
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Perspective |
Visualizing the genome in high resolution challenges our textbook understanding
This Perspective highlights how high-resolution imaging has informed our view and helped overturn the textbook understanding of 4D genome organization.
- Melike Lakadamyali
- & Maria Pia Cosma
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Research Highlight |
Variability in genome structure
Comparisons of genome organization in many individual cells with high-throughput FISH show extensive variation.
- Nicole Rusk
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Article |
Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads
Replication initiation is stochastic and obscured in population sequencing; D-NAscent reports the use of long nanopore reads to detect base analogs and thus to assess replication initiation at the individual molecule level.
- Carolin A. Müller
- , Michael A. Boemo
- & Conrad A. Nieduszynski
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Brief Communication |
CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
Multiple MS2 loops inserted in the first loop of an sgRNA after the spacer sequence stabilize the sgRNA and allow recruitment of multicolor fluorescent proteins for imaging of low-repeat genomic loci.
- Hanhui Ma
- , Li-Chun Tu
- & Thoru Pederson
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Research Highlight |
Does the number of chromosomes matter?
CRISPR–Cas9 enables successive fusions of 16 native chromosomes into one artificial single chromosome.
- Lei Tang
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Research Highlights |
Imaging allelic loci in live cells
SNP-CLING enables determining the dynamics of alleles on different chromosomes in live cells.
- Nina Vogt
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Methods in Brief |
Track first and identify later
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Research Highlights |
Finding function in the folds
Single-cell modeling of chromosomal organization could help scientists untangle how genomic organization informs function and vice versa.
- Michael Eisenstein
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Brief Communication |
Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation
iceFISH labels nascent RNA along a single chromosome, revealing chromosome territories and potential regulatory mechanisms working at the chromosome scale.
- Marshall J Levesque
- & Arjun Raj
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Methods in Brief |
Soft bacterial chromosomes
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Article |
Iterative correction of Hi-C data reveals hallmarks of chromosome organization
ICE (iterative correction and eigenvector decomposition) provides insight into inter- and intrachromosome interaction patterns.
- Maxim Imakaev
- , Geoffrey Fudenberg
- & Leonid A Mirny
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Research Highlights |
Finding the trees in the forest
The integration of quantitative proteomics and analysis by machine learning yields a refined list of proteins involved in chromosome function.
- Nicole Rusk
Exploring genomic data coupled with 3D chromatin structures using the WashU Epigenome Browser