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Article
| Open Access
DOT1L-mediated murine neuronal differentiation associates with H3K79me2 accumulation and preserves SOX2-enhancer accessibility
Neuronal differentiation requires rearrangement of the transcriptional and chromatin landscapes of neural cells. Here, the authors study in-vitro neuronal differentiation of murine embryonic stem cells (ESCs) to show that this process is modulated by DOT1L activity, which regulates H3K79me2 accumulation, and preserves accessibility of SOX2-bound enhancers.
- Francesco Ferrari
- , Laura Arrigoni
- & Thomas Manke
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Article
| Open Access
TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control
The HUSH complex plays a key role in controlling transcription of viruses and transposable elements. Here, the authors define the biochemical basis of HUSH assembly and show that the TASOR subunit contains a pseudo-PARP domain critical for HUSH-dependent transgene repression and H3K9me3 deposition over targets genome wide.
- Christopher H. Douse
- , Iva A. Tchasovnikarova
- & Yorgo Modis
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Article
| Open Access
Epigenetic specifications of host chromosome docking sites for latent Epstein-Barr virus
Epstein-Barr virus (EBV) episomes tether to the host chromosome via EBNA1. Here, using circular chromosome conformation capture (4C), Kim et al. identify attachment sites and show that EBV episomes preferentially associate with transcriptionally silenced genes in Burkitt lymphoma cells.
- Kyoung-Dong Kim
- , Hideki Tanizawa
- & Paul M. Lieberman
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Article
| Open Access
Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis
Meiotic DSB formation, repair and recombination occur in a continuum of substages termed leptonema, zygonema, pachynema, and diplonema. Here, authors develop a method for isolating pure sub-populations of nuclei that allows for detailed study of meiotic substages.
- Kwan-Wood Gabriel Lam
- , Kevin Brick
- & R. Daniel Camerini-Otero
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Article
| Open Access
Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells
In Drosophila the Male-Specific Lethal complex (MSLc) mediates upregulation of the single male X chromosome. Here the authors provide evidence that MSL2 also targets autosomal genes required for proper development and that MSL2 binds and similarly regulates mouse orthologues.
- Claudia Isabelle Keller Valsecchi
- , M. Felicia Basilicata
- & Asifa Akhtar
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Article
| Open Access
ChIP-seq reveals broad roles of SARD1 and CBP60g in regulating plant immunity
SARD1 and CBP60g are two plant transcription factors that regulate salicylic acid biosynthesis in response to pathogens. Here, Sun et al.show that they bind a wide array of loci related to multiple defence signalling pathways suggesting a broader role as regulators of the plant immune response.
- Tongjun Sun
- , Yaxi Zhang
- & Yuelin Zhang
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Article
| Open Access
Proteins that bind regulatory regions identified by histone modification chromatin immunoprecipitations and mass spectrometry
The protein factors that bind to regulatory regions in the genome have not been systematically mapped. Here the authors performed chromatin immunoprecipitations for histone modifications associated with promoters, enhancers or heterochromatin in mouse embryonic stem cells and assigned a genome location to many factors important for pluripotency.
- Erik Engelen
- , Johannes H. Brandsma
- & Raymond A. Poot
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An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations
Standard ChIP-seq protocols require large numbers of cells for high-quality datasets, limiting the application of this technique on rare cell types. Here, Brind’Amour et al. introduce an ultra-low-input ChIP-seq protocol to generate maps of covalent histone marks from as few as 1,000 cells.
- Julie Brind’Amour
- , Sheng Liu
- & Matthew C. Lorincz
Genome-wide binding potential and regulatory activity of the glucocorticoid receptor’s monomeric and dimeric forms