Chromatin immunoprecipitation

Definition

Chromatin immunoprecipitation (ChIP) is a technique for identifying DNA sequences associated with specific proteins in vivo. The association of DNA fragments with a binding protein, such as a transcription factor or modified histone, can be assessed by sequencing, microarrays or PCR after precipitation with an antibody that recognizes the protein.

Latest Research and Reviews

  • Reviews |

    A full understanding of chromatin in diverse cellular processes requires the consideration of its dynamics, but most standard chromatin assays provide only a static snapshot. This Review describes various emerging methods for probing chromatin dynamics across a wide range of temporal and spatial scales, and discusses the resulting biological insights.

    • Olivier Cuvier
    •  & Beat Fierz
  • Research |

    Margaret Goodell, Wei Li and colleagues use double-knockout mice for Dnmt3a and Tet2 to model leukemia development. Through epigenetic and transcriptional analyses, they show that loss of DNMT3A and TET2 upregulates lineage-specific transcription factors such as KLF1 in hematopoietic stem cells and accelerates malignancy.

    • Xiaotian Zhang
    • , Jianzhong Su
    • , Mira Jeong
    • , Myunggon Ko
    • , Yun Huang
    • , Hyun Jung Park
    • , Anna Guzman
    • , Yong Lei
    • , Yung-Hsin Huang
    • , Anjana Rao
    • , Wei Li
    •  & Margaret A Goodell
    Nature Genetics 48, 1014–1023
  • Research |

    Genomic studies of the paediatric brain tumour medulloblastoma have revealed four clinically distinct molecular subgroups; here active gene regulatory elements in 28 primary medulloblastoma tissues are mapped to reveal differentially regulated enhancers across the different subgroups, allowing insights into the transcription factors that characterize subgroup divergence and the cellular origin of the poorly characterized Group 3 and 4 subgroups.

    • Charles Y. Lin
    • , Serap Erkek
    • , Yiai Tong
    • , Linlin Yin
    • , Alexander J. Federation
    • , Marc Zapatka
    • , Parthiv Haldipur
    • , Daisuke Kawauchi
    • , Thomas Risch
    • , Hans-Jörg Warnatz
    • , Barbara C. Worst
    • , Bensheng Ju
    • , Brent A. Orr
    • , Rhamy Zeid
    • , Donald R. Polaski
    • , Maia Segura-Wang
    • , Sebastian M. Waszak
    • , David T. W. Jones
    • , Marcel Kool
    • , Volker Hovestadt
    • , Ivo Buchhalter
    • , Laura Sieber
    • , Pascal Johann
    • , Lukas Chavez
    • , Stefan Gröschel
    • , Marina Ryzhova
    • , Andrey Korshunov
    • , Wenbiao Chen
    • , Victor V. Chizhikov
    • , Kathleen J. Millen
    • , Vyacheslav Amstislavskiy
    • , Hans Lehrach
    • , Marie-Laure Yaspo
    • , Roland Eils
    • , Peter Lichter
    • , Jan O. Korbel
    • , Stefan M. Pfister
    • , James E. Bradner
    •  & Paul A. Northcott
    Nature 530, 57–62

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