Featured
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Method to Watch |
Tracing cell relationships
Lineage tracing methods help unravel complexities of cell state and differentiation programs.
- Madhura Mukhopadhyay
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Research Highlight |
Metabolic profiling of CD8+ T cells at the single-cell level
A single-cell approach to metabolomics links metabolic profiles to cellular identity.
- Madhura Mukhopadhyay
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Article |
Detecting chromatin interactions between and along sister chromatids with SisterC
It remains impossible using conventional Hi-C to differentiate interactions between and along sister chromatids. SisterC relies on selective destruction of nascent DNA and in combination with Hi-C offers a means to study intra- and inter-sister interactions independently.
- Marlies E. Oomen
- , Adam K. Hedger
- & Job Dekker
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Article |
Noninvasive monitoring of single-cell mechanics by acoustic scattering
Acoustic scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.
- Joon Ho Kang
- , Teemu P. Miettinen
- & Scott R. Manalis
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Brief Communication |
FLIRT: fast local infrared thermogenetics for subcellular control of protein function
FLIRT enables spatiotemporally precise control of protein function in C. elegans by harnessing existing temperature-sensitive mutations. Proteins can be inactivated at desired sites by infrared laser light targeted to the region(s) of interest.
- Sophia M. Hirsch
- , Sriramkumar Sundaramoorthy
- & Julie C. Canman
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Research Highlight |
Toward a 3D genome in high resolution
Independent efforts shine light on the 3D genome structure by looking at multiple contacts along an allele or equalizing the distance between restriction sites for higher-resolution Hi-C maps.
- Nicole Rusk
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Tools in Brief |
Optimized light-dependent dimerization
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Article |
Oscillatory stimuli differentiate adapting circuit topologies
Negative feedback and incoherent feed-forward loops are the only circuit motifs that can adapt in response to stimuli. Rahi et al. describe signatures that allow discriminating between these two motifs and demonstrate the approach in yeast cell cycle timing and C. elegans olfaction.
- Sahand Jamal Rahi
- , Johannes Larsch
- & Frederick R Cross
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Tools in Brief |
An all-in-one cell cycle reporter
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Brief Communication |
Fluorescent indicators for simultaneous reporting of all four cell cycle phases
The far-red fluorescent protein mMaroon1 and a reporter based on stem-loop binding protein enables the generation of Fucci4, a 4-color cell cycle reporter system that can be used to distinguish all phases of the cell cycle. Also online, a paper by Laviv et al. uses mMaroon1 as a FRET acceptor for the newly developed CyRFP1.
- Bryce T Bajar
- , Amy J Lam
- & Michael Z Lin
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Research Highlights |
A nervous system from Mom and a gonad from Dad
A simple tweak on the zygote's mitotic spindle generated hybrid Caenorhabditis elegans with certain tissues (such as those of the nervous system) consisting of entirely maternal genomes and others (such as the germline) consisting of entirely paternal genomes.
- Kate Gao
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Article |
High-density three-dimensional localization microscopy across large volumes
Lattice light-sheet and PAINT microscopy are combined to achieve low-background detection of dense molecular labels, yielding super-resolution localization microscopy images of intricate 3D structures within dividing cells and embryos.
- Wesley R Legant
- , Lin Shao
- & Eric Betzig
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Tools in Brief |
Label-free image flow cytometry
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Brief Communication |
Trajectories of cell-cycle progression from fixed cell populations
Cycler constructs a trajectory of cell-cycle progression from fixed images of cells enabling the correlation of an individual cell's position in the cell cycle with multiple cellular readouts.
- Gabriele Gut
- , Michelle D Tadmor
- & Prisca Liberali
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This Month |
Stefan Florian
Tracking cell cycles in vivo, and the temptation of science fiction.
- Vivien Marx
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Article |
In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy
Methods for quantitative, automated in vivo cell-cycle profiling are applied to tumor xenografts in the mouse to study the effects of drug treatment.
- Deepak R Chittajallu
- , Stefan Florian
- & Timothy J Mitchison
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Tools in Brief |
A multifocal approach to whole-cell super-resolution imaging
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Perspective |
Live-cell mass profiling: an emerging approach in quantitative biophysics
This Perspective discusses methods to measure single-cell mass and their relative strengths and weaknesses for different applications.
- Thomas A Zangle
- & Michael A Teitell
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Methods in Brief |
Live-cell automated super-resolution microscopy
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Research Highlights |
A brief history of nuclear organization
By marking protein-DNA interactions in real time, scientists track chromosomal rearrangements in the nuclei of living cells.
- Michael Eisenstein
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Brief Communication |
A simple tool to improve pluripotent stem cell differentiation
Treatment with DMSO improves the differentiation of multiple human pluripotent stem cell lines into cells of all three germ layers.
- Sundari Chetty
- , Felicia Walton Pagliuca
- & Douglas A Melton
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Research Highlights |
Discovering Mozart
High-throughput imaging of genome-wide RNA interference screens and systematic analysis of protein complexes involved in mitosis yield valuable resources and reveal new subunits of well-characterized complexes.
- Nicole Rusk
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Research Highlights |
Immortal strands versus silent sisters
A method to differentially label each sister chromatid in a cell makes it possible to elucidate segregation patterns after mitosis and should help to pinpoint the mechanism behind nonrandom segregation in certain cell types.
- Nicole Rusk
Cell region fingerprints enable highly precise single-cell tracking and lineage reconstruction