Biophysics

  • Article
    | Open Access

    The design of protein assemblies is a major thrust for biomolecular engineering and nanobiotechnology. Here the authors demonstrate a general mechanism for designing allosteric macromolecular assemblies and showcase a proof of concept for engineered allosteric protein assembly.

    • Luis A. Campos
    • , Rajendra Sharma
    • , Sara Alvira
    • , Federico M. Ruiz
    • , Beatriz Ibarra-Molero
    • , Mourad Sadqi
    • , Carlos Alfonso
    • , Germán Rivas
    • , Jose M. Sanchez-Ruiz
    • , Antonio Romero Garrido
    • , José M. Valpuesta
    •  & Victor Muñoz
  • Article
    | Open Access

    Glioblastoma cells are known to be able to adapt easily to different environments. The authors study the dynamic adaptation of glioblastoma cells to the heterogenous brain tumor microenvironment, showing that tumor cells demonstrate varying plasticity of their transcriptomic profiles and an ability to survive new stimuli, in part, by propagating stochastic perturbations over their gene-regulatory network.

    • Orieta Celiku
    • , Mark R. Gilbert
    •  & Orit Lavi
  • Article
    | Open Access

    Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-synuclein aggregation.

    • James W. P. Brown
    • , Arnaud Bauer
    • , Mark E Polinkovsky
    • , Akshay Bhumkar
    • , Dominic J. B. Hunter
    • , Katharina Gaus
    • , Emma Sierecki
    •  & Yann Gambin
  • Article
    | Open Access

    Engineered biological nanopores enable observation of single molecule chemistry events; however a cylindrical pore geometry can have undesired effects. The authors report a conical biological pore which was embedded with tetrachloroaurate(III) to allow for discrimination between different biothiols.

    • Jiao Cao
    • , Wendong Jia
    • , Jinyue Zhang
    • , Xiumei Xu
    • , Shuanghong Yan
    • , Yuqin Wang
    • , Panke Zhang
    • , Hong-Yuan Chen
    •  & Shuo Huang
  • Article
    | Open Access

    Artificial nanopores can perform selective gating of molecules analogous to transmembrane proteins. Here, the authors design a DNA origami pore with a controllable lid for size-selective gating and translocation of macromolecules and evaluate its biosensing properties by single particle assay.

    • Rasmus P. Thomsen
    • , Mette Galsgaard Malle
    • , Anders Hauge Okholm
    • , Swati Krishnan
    • , Søren S.-R. Bohr
    • , Rasmus Schøler Sørensen
    • , Oliver Ries
    • , Stefan Vogel
    • , Friedrich C. Simmel
    • , Nikos S. Hatzakis
    •  & Jørgen Kjems
  • Article
    | Open Access

    PARG catalyzes the removal of poly(ADP-ribose) (PAR) from target proteins and executes critical functions in the DNA damage response. Here the authors provide structural and biological insight with small molecule PARG inhibitors and show that PARG inhibition sensitizes cells to ionizing radiation and kills cancer cells through replication fork defects.

    • Jerry H. Houl
    • , Zu Ye
    • , Chris A. Brosey
    • , Lakshitha P. F. Balapiti-Modarage
    • , Sarita Namjoshi
    • , Albino Bacolla
    • , Daniel Laverty
    • , Brian L. Walker
    • , Yasin Pourfarjam
    • , Leslie S. Warden
    • , Naga Babu Chinnam
    • , Davide Moiani
    • , Roderick A. Stegeman
    • , Mei-Kuang Chen
    • , Mien-Chie Hung
    • , Zachary D. Nagel
    • , Tom Ellenberger
    • , In-Kwon Kim
    • , Darin E. Jones
    • , Zamal Ahmed
    •  & John A. Tainer
  • Article
    | Open Access

    Multiple sequence alignments of proteins carry information about evolution, the protein’s fitness landscape and its stability in the face of mutations. Here, the authors demonstrate the utility of latent space models learned using variational autoencoders to infer these properties from sequences.

    • Xinqiang Ding
    • , Zhengting Zou
    •  & Charles L. Brooks III
  • Article
    | Open Access

    Increasing evidence supports the existence of ordered nanodomains (or rafts) in cholesterol rich cell membranes. Here authors present molecular dynamics simulations and EPR experiments to monitor permeation of oxygen and water through membranes in the liquid ordered and liquid disordered phases.

    • An Ghysels
    • , Andreas Krämer
    • , Richard M. Venable
    • , Walter E. Teague Jr
    • , Edward Lyman
    • , Klaus Gawrisch
    •  & Richard W. Pastor
  • Article
    | Open Access

    Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.

    • Yanjun Zhang
    • , Yasufumi Takahashi
    • , Sung Pil Hong
    • , Fengjie Liu
    • , Joanna Bednarska
    • , Philip S. Goff
    • , Pavel Novak
    • , Andrew Shevchuk
    • , Sahana Gopal
    • , Iros Barozzi
    • , Luca Magnani
    • , Hideki Sakai
    • , Yoshimoto Suguru
    • , Takuto Fujii
    • , Alexander Erofeev
    • , Peter Gorelkin
    • , Alexander Majouga
    • , Dominik J. Weiss
    • , Christopher Edwards
    • , Aleksandar P. Ivanov
    • , David Klenerman
    • , Elena V. Sviderskaya
    • , Joshua B. Edel
    •  & Yuri Korchev
  • Article
    | Open Access

    Metabotropic glutamate receptors (mGluRs) are dimeric G-protein–coupled receptors that operate at neural synapses. Here authors use FRET assays in live cells to monitor mGluR2/7’s activation and reveal how heterodimerization can alter the glutamate response of an mGluR.

    • Chris H. Habrian
    • , Joshua Levitz
    • , Vojtech Vyklicky
    • , Zhu Fu
    • , Adam Hoagland
    • , Isabelle McCort-Tranchepain
    • , Francine Acher
    •  & Ehud Y. Isacoff
  • Article
    | Open Access

    Wireframe DNA origami is a powerful approach to creating 2D and 3D geometries. Here the authors introduce an automated computational design approach that programs structures with high structural fidelity.

    • Hyungmin Jun
    • , Xiao Wang
    • , William P. Bricker
    •  & Mark Bathe
  • Article
    | Open Access

    The human ATP-binding cassette transporter ABCG2 plays critical roles in anticancer resistance but the molecular mechanism of ABCG2-mediated substrate transport remains enigmatic. Here authors use extensive mutagenesis and molecular dynamics simulations to reveal a mechanistic basis for the function of the di-leucine valve and the roof organization in the transport cycle.

    • Narakorn Khunweeraphong
    • , Daniel Szöllősi
    • , Thomas Stockner
    •  & Karl Kuchler
  • Article
    | Open Access

    Cytotoxic T lymphocytes (CTLs) eliminate virus-infected and cancerous cells by secreting the pore-forming protein (perforin) and pro-apoptotic serine proteases (granzymes). Here authors show that two mechanisms protect the membranes of CTLs from disruption by perforin and granzymes.

    • Jesse A. Rudd-Schmidt
    • , Adrian W. Hodel
    • , Tahereh Noori
    • , Jamie A. Lopez
    • , Hyun-Jung Cho
    • , Sandra Verschoor
    • , Annette Ciccone
    • , Joseph A. Trapani
    • , Bart W. Hoogenboom
    •  & Ilia Voskoboinik
  • Article
    | Open Access

    Swimming bacteria perform collective motion at high cell density, yet it is unclear how this behaviour affects their ability to follow substance gradients in the environment. Here, Colin et al. address this question by studying motion of Escherichia coli in controlled chemical gradients.

    • Remy Colin
    • , Knut Drescher
    •  & Victor Sourjik
  • Article
    | Open Access

    The endoplasmic reticulum (ER) is an intracellular network characterized by highly dynamic behavior whose control mechanisms are unclear. Here, the authors show that the ER-membrane protein Reticulon (Rtnl1) can constrict ER bilayers and lead to ER fission.

    • Javier Espadas
    • , Diana Pendin
    • , Rebeca Bocanegra
    • , Artur Escalada
    • , Giulia Misticoni
    • , Tatiana Trevisan
    • , Ariana Velasco del Olmo
    • , Aldo Montagna
    • , Sergio Bova
    • , Borja Ibarra
    • , Peter I. Kuzmin
    • , Pavel V. Bashkirov
    • , Anna V. Shnyrova
    • , Vadim A. Frolov
    •  & Andrea Daga
  • Article
    | Open Access

    The bacterial flagellar hook is a molecular universal joint that connects the rotary motor and long helical propeller of the bacterial flagellum. Here the authors present the 3.6 Å resolution cryo-EM structure of the native supercoiled Salmonella hook that provides insights into the dynamic changes of subunit conformations and intermolecular interactions of the hook protein FlgE.

    • Takayuki Kato
    • , Fumiaki Makino
    • , Tomoko Miyata
    • , Péter Horváth
    •  & Keiichi Namba
  • Article
    | Open Access

    The mechanics of MMR strand specific excision that begins at a distant ssDNA break are not yet clear. Here the authors have used multiple single molecule imaging techniques to visualize the behavior of MMR components on mismatched DNA substrates and reveal an exonuclease-independent mechanism for E.coli MMR.

    • Jiaquan Liu
    • , Ryanggeun Lee
    • , Brooke M. Britton
    • , James A. London
    • , Keunsang Yang
    • , Jeungphill Hanne
    • , Jong-Bong Lee
    •  & Richard Fishel
  • Article
    | Open Access

    The mechanisms regulating the turnover of the AMPARs in the synapse, which is critically important to sustain basic synaptic activity, remains unclear. In this study, authors used single-molecule imaging techniques to demonstrate that AMPAR tetramers are not stable entities and readily fall apart to dimers and monomers that could reform to tetramers at the synapse, and that rapidly diffusing monomers in the plasma membrane are primarily responsible for the AMPAR turnover in the synapse.

    • Jyoji Morise
    • , Kenichi G. N. Suzuki
    • , Ayaka Kitagawa
    • , Yoshihiko Wakazono
    • , Kogo Takamiya
    • , Taka A. Tsunoyama
    • , Yuri L. Nemoto
    • , Hiromu Takematsu
    • , Akihiro Kusumi
    •  & Shogo Oka
  • Article
    | Open Access

    Former evidence suggests a correlation between the function of non-conventional myosin motors and actin dynamics. Here authors use in vitro assays in which they observe that actin sliding on myosin 1b immobilized or bound to a fluid bilayer enhances actin depolymerization at the barbed end.

    • Julien Pernier
    • , Remy Kusters
    • , Hugo Bousquet
    • , Thibaut Lagny
    • , Antoine Morchain
    • , Jean-François Joanny
    • , Patricia Bassereau
    •  & Evelyne Coudrier
  • Article
    | Open Access

    Active translocation of DNA through nanopores usually needs enzyme assistance. Here authors present a nanopore derived from helicase E1 of bovine papillomavirus (BPV) which acts as a conductive pore embedded in lipid membrane to allow the translocation of ssDNA and unwinding of dsDNA.

    • Ke Sun
    • , Changjian Zhao
    • , Xiaojun Zeng
    • , Yuejia Chen
    • , Xin Jiang
    • , Xianting Ding
    • , Lu Gou
    • , Haiyang Xie
    • , Xinqiong Li
    • , Xialin Zhang
    • , Sheng Lin
    • , Linqin Dou
    • , Long Wei
    • , Haofu Niu
    • , Ming Zhang
    • , Ruocen Tian
    • , Erica Sawyer
    • , Qingyue Yuan
    • , Yuqin Huang
    • , Piaopiao Chen
    • , Chengjian Zhao
    • , Cuisong Zhou
    • , Binwu Ying
    • , Bingyang Shi
    • , Xiawei Wei
    • , Ruotian Jiang
    • , Lei Zhang
    • , Guangwen Lu
    •  & Jia Geng
  • Article
    | Open Access

    Magnetoencephalography (MEG) recordings are sensitive to movement and therefore are especially challenging with young participants. Here the authors develop a wearable MEG system based on a modified bicycle helmet, which enables reliable recordings in toddlers, children, teenagers and adults.

    • Ryan M. Hill
    • , Elena Boto
    • , Niall Holmes
    • , Caroline Hartley
    • , Zelekha A. Seedat
    • , James Leggett
    • , Gillian Roberts
    • , Vishal Shah
    • , Tim M. Tierney
    • , Mark W. Woolrich
    • , Charlotte J. Stagg
    • , Gareth R. Barnes
    • , Richard Bowtell
    • , Rebeccah Slater
    •  & Matthew J. Brookes
  • Article
    | Open Access

    Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Here authors find and structurally characterize the selective interaction between CLCa and the actin motor protein myosin VI which act together to generate the force that leads to invagination and fission at the apical surface.

    • Matteo Biancospino
    • , Gwen R. Buel
    • , Carlos A. Niño
    • , Elena Maspero
    • , Rossella Scotto di Perrotolo
    • , Andrea Raimondi
    • , Lisa Redlingshöfer
    • , Janine Weber
    • , Frances M. Brodsky
    • , Kylie J. Walters
    •  & Simona Polo
  • Article
    | Open Access

    A membrane-exposed groove in Ca2+-gated TMEM16 scramblases forms the translocation pathway for ions and lipids. Here authors combine molecular dynamics with cryo-EM and functional assays to uncover the conformational transitions of the groove leading to a non-selective ion channel pore.

    • George Khelashvili
    • , Maria E. Falzone
    • , Xiaolu Cheng
    • , Byoung-Cheol Lee
    • , Alessio Accardi
    •  & Harel Weinstein
  • Article
    | Open Access

    Rhodopsin genes have been identified in some large double-stranded DNA viruses, but the structure and functions of viral rhodopsins remain unknown. Here authors present crystal structure and characterization of an Organic Lake Phycodnavirus rhodopsin II (OLPVRII) which forms a pentamer and is a weak proton pump.

    • Dmitry Bratanov
    • , Kirill Kovalev
    • , Jan-Philipp Machtens
    • , Roman Astashkin
    • , Igor Chizhov
    • , Dmytro Soloviov
    • , Dmytro Volkov
    • , Vitaly Polovinkin
    • , Dmitrii Zabelskii
    • , Thomas Mager
    • , Ivan Gushchin
    • , Tatyana Rokitskaya
    • , Yuri Antonenko
    • , Alexey Alekseev
    • , Vitaly Shevchenko
    • , Natalya Yutin
    • , Riccardo Rosselli
    • , Christian Baeken
    • , Valentin Borshchevskiy
    • , Gleb Bourenkov
    • , Alexander Popov
    • , Taras Balandin
    • , Georg Büldt
    • , Dietmar J. Manstein
    • , Francisco Rodriguez-Valera
    • , Christoph Fahlke
    • , Ernst Bamberg
    • , Eugene Koonin
    •  & Valentin Gordeliy
  • Article
    | Open Access

    The structures and dynamics of large proteins can be studied with methyl-based NMR but peak assignment is still challenging. Here the authors present MethylFLYA that allows automated assignment of methyl groups and apply it to five proteins with molecular weights in the range from 28 to 358 kDa.

    • Iva Pritišanac
    • , Julia M. Würz
    • , T. Reid Alderson
    •  & Peter Güntert
  • Article
    | Open Access

    Elastic mapping of individual layers of the cornea with elastography uses Lamb waves, which are dependent on the thickness of each layer and the direction of propagation. Here the authors present Reverberant 3D Optical Coherence Elastography to measure elasticity of single layers using waves propagating in all directions.

    • Fernando Zvietcovich
    • , Pornthep Pongchalee
    • , Panomsak Meemon
    • , Jannick P. Rolland
    •  & Kevin J. Parker
  • Article
    | Open Access

    Soft porous crystals hold big promise as functional nanoporous materials due to their stimuli responsive flexibility. Here, molecular dynamics simulations reveal a new type of spatial disorder in mesoscale crystals that helps to understand the size-dependency of their phase transition behavior.

    • Sven M. J. Rogge
    • , Michel Waroquier
    •  & Veronique Van Speybroeck
  • Article
    | Open Access

    Natural creatures, from fish to snake and birds, combine neural control, sensory feedback and compliant mechanics to operate across uncertain environments. Here the authors present a versatile modeling approach to the dynamic simulation of their architectures based on the assembly of Cosserat rods.

    • Xiaotian Zhang
    • , Fan Kiat Chan
    • , Tejaswin Parthasarathy
    •  & Mattia Gazzola
  • Article
    | Open Access

    Spy is an ATP independent chaperone that allows folding of its client protein Im7 while continuously bound to Spy. Here the authors employ kinetics measurements to study the folding of another Spy client protein SH3 and find that Spy’s ability to allow a client to fold while bound is inversely related to how strongly it interacts with that client.

    • Kevin Wu
    • , Frederick Stull
    • , Changhan Lee
    •  & James C. A. Bardwell
  • Article
    | Open Access

    Myosin, a motor protein essential for intracellular transport to muscle contraction, requires a chaperone UNC-45 for folding and assembly. Here authors use in vitro reconstitution and structural biology to characterize the interplay between UNC-45 and muscle myosin MHC-B.

    • Doris Hellerschmied
    • , Anita Lehner
    • , Nina Franicevic
    • , Renato Arnese
    • , Chloe Johnson
    • , Antonia Vogel
    • , Anton Meinhart
    • , Robert Kurzbauer
    • , Luiza Deszcz
    • , Linn Gazda
    • , Michael Geeves
    •  & Tim Clausen
  • Article
    | Open Access

    Podosomes are protrusive structures that coordinate diverse functions related to cell invasion, migration, bone resorption and immune surveillance. Here the authors integrate DNA nanotechnology with FLIM-FRET to demonstrate that podosomes apply pN integrin tensile forces to sense and respond to substrate mechanics.

    • Roxanne Glazier
    • , Joshua M. Brockman
    • , Emily Bartle
    • , Alexa L. Mattheyses
    • , Olivier Destaing
    •  & Khalid Salaita
  • Article
    | Open Access

    The Bin/Amphiphysin/Rvs167 (BAR) domain superfamily, which includes FCH-BAR (F-BAR) domain proteins are membrane-sculpting proteins. Here the authors combine a range of techniques and show that the F-BAR domain of growth-arrest specific protein 7 (GAS7) forms two-dimensional sheets on flat membranes and that these oligomeric assemblies of GAS7 are required for the formation of phagocytic cups in macrophages.

    • Kyoko Hanawa-Suetsugu
    • , Yuzuru Itoh
    • , Maisarah Ab Fatah
    • , Tamako Nishimura
    • , Kazuhiro Takemura
    • , Kohei Takeshita
    • , Satoru Kubota
    • , Naoyuki Miyazaki
    • , Wan Nurul Izzati Wan Mohamad Noor
    • , Takehiko Inaba
    • , Nhung Thi Hong Nguyen
    • , Sayaka Hamada-Nakahara
    • , Kayoko Oono-Yakura
    • , Masashi Tachikawa
    • , Kenji Iwasaki
    • , Daisuke Kohda
    • , Masaki Yamamoto
    • , Akio Kitao
    • , Atsushi Shimada
    •  & Shiro Suetsugu
  • Article
    | Open Access

    Retroviral integration of DNA into the host genome is a point of no return in the replication cycle but how efficient integration can take place remains unclear. Here the authors demonstrate that consecutive nucleoprotein intermediates are increasingly stable, resulting in a net forward rate.

    • Willem Vanderlinden
    • , Tine Brouns
    • , Philipp U. Walker
    • , Pauline J. Kolbeck
    • , Lukas F. Milles
    • , Wolfgang Ott
    • , Philipp C. Nickels
    • , Zeger Debyser
    •  & Jan Lipfert
  • Article
    | Open Access

    Biology serves as inspiration in materials development; this requires improved understanding of the surface chemistry responsible for processes which are being mimicked. Here, the authors report on the use of near edge X-ray absorption fine structure (NEXAFS) imaging to analyze the surface chemistry of insect cuticle.

    • Joe E. Baio
    • , Cherno Jaye
    • , Erin Sullivan
    • , Mette H. Rasmussen
    • , Daniel A. Fischer
    • , Stanislav Gorb
    •  & Tobias Weidner
  • Article
    | Open Access

    Mutations in the chloride channel ClC-2 have been found in primary aldosteronism (PA). Here, Göppner et al. generate transgenic mice expressing a mutant form of ClC-2 that displays increased chloride currents like patient mutations, and find it recapitulates the key pathological features of PA.

    • Corinna Göppner
    • , Ian J. Orozco
    • , Maja B. Hoegg-Beiler
    • , Audrey H. Soria
    • , Christian A. Hübner
    • , Fabio L. Fernandes-Rosa
    • , Sheerazed Boulkroun
    • , Maria-Christina Zennaro
    •  & Thomas J. Jentsch
  • Article
    | Open Access

    Understanding vibrational energy transfer in macromolecules has been challenging to both theory and experiment. Here the authors use non-equilibrium molecular dynamics to reveal the relationship between heat transport in a model peptide, emergent nonlinearity, and the underlying free energy landscape.

    • Justin E. Elenewski
    • , Kirill A. Velizhanin
    •  & Michael Zwolak
  • Article
    | Open Access

    How mechanosensitive ion channels, such as MscL, are activated by lipids and physical properties of the membrane remains unclear. Here authors use PELDOR/DEER spectroscopy and identify a single site which generated an allosteric structural response in the absence of membrane tension.

    • Charalampos Kapsalis
    • , Bolin Wang
    • , Hassane El Mkami
    • , Samantha J. Pitt
    • , Jason R. Schnell
    • , Terry K. Smith
    • , Jonathan D. Lippiat
    • , Bela E. Bode
    •  & Christos Pliotas
  • Article
    | Open Access

    Programmed ribosomal frameshifting (PRF) is an alternative translation strategy that causes controlled slippage of the ribosome along the mRNA, changing the sequence of the synthesized protein. Here the authors provide a thermodynamic framework that explains how mRNA sequence determines the efficiency of frameshifting.

    • Lars V. Bock
    • , Neva Caliskan
    • , Natalia Korniy
    • , Frank Peske
    • , Marina V. Rodnina
    •  & Helmut Grubmüller
  • Article
    | Open Access

    The interaction between proteins and nanomaterials is complex and of interest for controlling nanoparticle fate. Here, using experimental and computational methods, the authors report on the effect of hydroxyl groups on protein interaction and how they can be used to enhance circulation times.

    • Xiang Lu
    • , Peipei Xu
    • , Hong-Ming Ding
    • , You-Sheng Yu
    • , Da Huo
    •  & Yu-Qiang Ma
  • Article
    | Open Access

    Processing bodies are membrane less organelles that contain enzymes involved in mRNA turnover, among them enhancer of decapping 3 (Edc3). Here the authors use solid- and solution-state NMR spectroscopy to characterize the structural organization and dynamics of Edc3 and find that its interactions with RNA and between the different Edc3 domains are largely preserved in the phase-separated state.

    • Reinier Damman
    • , Stefan Schütz
    • , Yanzhang Luo
    • , Markus Weingarth
    • , Remco Sprangers
    •  & Marc Baldus
  • Article
    | Open Access

    Besides mechanical forces, the mechanosensitive channel Piezo1 is activated by the small molecule Yoda1 through an unknown mechanism. Here, using molecular dynamics simulations, calcium imaging and electrophysiology, the authors identify an allosteric Yoda1 binding pocket located in the putative mechanosensory domain.

    • Wesley M. Botello-Smith
    • , Wenjuan Jiang
    • , Han Zhang
    • , Alper D. Ozkan
    • , Yi-Chun Lin
    • , Christine N. Pham
    • , Jérôme J. Lacroix
    •  & Yun Luo
  • Article
    | Open Access

    Nanopore microscopy is capable of detecting knots in DNA polymers, a common structural feature. Here the authors show nanopore sensors can map the equilibrium structure of these knots between tight and loose states.

    • Rajesh Kumar Sharma
    • , Ishita Agrawal
    • , Liang Dai
    • , Patrick S. Doyle
    •  & Slaven Garaj
  • Article
    | Open Access

    The reovirus outercapsid protein σ1 binds to α-linked sialic acid and junctional adhesion molecule A (JAM-A) for virus entry. Here, combining atomic force microscopy and confocal microscopy, the authors provide insights into the dynamics of these interactions at the single-virion level in living cells.

    • Melanie Koehler
    • , Pavithra Aravamudhan
    • , Camila Guzman-Cardozo
    • , Andra C. Dumitru
    • , Jinsung Yang
    • , Serena Gargiulo
    • , Patrice Soumillion
    • , Terence S. Dermody
    •  & David Alsteens
  • Article
    | Open Access

    Viruses have evolved protein containers with a wide spectrum of icosahedral architectures but the geometric constraints defining these container designs remain to be understood. Here authors revisit the construction of icosahedral architectures using the Archimedean lattices that explain the outliers to the current classification scheme.

    • Reidun Twarock
    •  & Antoni Luque
  • Article
    | Open Access

    Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and slides along the DNA during target search.

    • Tao Ju Cui
    • , Misha Klein
    • , Jorrit W. Hegge
    • , Stanley D. Chandradoss
    • , John van der Oost
    • , Martin Depken
    •  & Chirlmin Joo
  • Article
    | Open Access

    The molecular identity of the mitochondrial megachannel (MMC)/permeability transition pore (PTP), a key effector of cell death, remains controversial. Here authors demonstrate that the membrane embedded bovine F-ATP synthase elicits Ca2 + -dependent currents matching those of the MMC/PTP.

    • Andrea Urbani
    • , Valentina Giorgio
    • , Andrea Carrer
    • , Cinzia Franchin
    • , Giorgio Arrigoni
    • , Chimari Jiko
    • , Kazuhiro Abe
    • , Shintaro Maeda
    • , Kyoko Shinzawa-Itoh
    • , Janna F. M. Bogers
    • , Duncan G. G. McMillan
    • , Christoph Gerle
    • , Ildikò Szabò
    •  & Paolo Bernardi
  • Article
    | Open Access

    Munc18-1 and Munc13-1 are key for the exquisite regulation of neurotransmitter release. Here biophysical experiments show how αSNAP inhibits liposome fusion mediated by the neuronal SNAREs and how Munc18-1 overcomes this inhibition, ensuring that release depends on Munc18-1 and Munc13-1.

    • Karolina P. Stepien
    • , Eric A. Prinslow
    •  & Josep Rizo