Assay systems articles within Nature

Featured

• Article | 14 October 2020

  Dense and pleiotropic regulatory information in a developmental enhancer

  A robotic pipeline is used to survey a library of mutations in a Drosphila gene enhancer, showing that most mutations altered gene expression and had widespread pleiotropic effects that are likely to constrain regulatory evolution.

  • Timothy Fuqua
  • , Jeff Jordan
  •  & Justin Crocker

• Article | 30 October 2019

  Allele-selective lowering of mutant HTT protein by HTT–LC3 linker compounds

  Compounds that interact with mutant huntingtin and an autophagosomal protein are able to reduce cellular levels of mutant huntingtin by targeting it for autophagic degradation, demonstrating an approach that may have potential for treating proteopathies.

  • Zhaoyang Li
  • , Cen Wang
  •  & Boxun Lu

• Letter | 12 September 2018

  In vivo CRISPR editing with no detectable genome-wide off-target mutations

  A strategy developed to define off-target effects of gene-editing nucleases in whole organisms is validated and leveraged to show that CRISPR–Cas9 nucleases can be used effectively in vivo without inducing detectable off-target mutations.

  • Pinar Akcakaya
  • , Maggie L. Bobbin
  •  & J. Keith Joung

• Article | 01 August 2016

  Circadian neuron feedback controls the Drosophila sleep–activity profile

  A subset of dorsal clock neurons are identified in Drosophila as sleep-promoting cells, which participate in a feedback loop with pacemaker neurons to drive both midday siesta and night-time sleep.

  • Fang Guo
  • , Junwei Yu
  •  & Michael Rosbash