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Involvement of upregulation of DEPDC1 (DEP domain containing 1) in bladder carcinogenesis

Oncogene volume 26, pages 6448–6455 (2007)Cite this article

Abstract

In an attempt to disclose mechanisms of bladder carcinogenesis and discover novel target molecules for development of treatment, we applied a cDNA microarray to screen genes that were significantly transactivated in bladder cancer cells. Among the upregulated genes, we here focused on a novel gene, (DEPDC1) DEP domain containing 1, whose overexpression was confirmed by northern blot and immunohistochemical analyses. Immunocytochemical staining analysis detected strong staining of endogenous DEPDC1 protein in the nucleus of bladder cancer cells. Since DEPDC1 expression was hardly detectable in any of 24 normal human tissues we examined except the testis, we considered this gene-product to be a novel cancer/testis antigen. Suppression of DEPDC1 expression with small-interfering RNA significantly inhibited growth of bladder cancer cells. Taken together, these findings suggest that DEPDC1 might play an essential role in the growth of bladder cancer cells, and would be a promising molecular-target for novel therapeutic drugs or cancer peptide-vaccine to bladder cancers.

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Acknowledgements

We thank Ms Yoshiko Fujisawa and Ms Kie Naito, for grateful technical supports and Dr Yataro Daigo for preparation of lung tissues and Dr Shingo Ashida of Department of Urology, Kochi Medical School and Dr Wataru Obara of Department of Urology, Iwate Medical University for preparation of bladder cancer tissues sections, and Dr Akira Togashi for helpful discussion.

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Author notes

  1. M Kanehira and Y Harada: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan

    M Kanehira, Y Harada, R Takata, Y Nakamura & T Katagiri

  2. Department of Urology, Iwate Medical University, Morioka, Japan

    M Kanehira, R Takata & T Fujioka

  3. Department of Urology, Kochi Medical School, Nankoku, Japan

    T Shuin

  4. Department of Urology, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan

    T Miki

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  1. M Kanehira
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Correspondence to T Katagiri.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Kanehira, M., Harada, Y., Takata, R. et al. Involvement of upregulation of DEPDC1 (DEP domain containing 1) in bladder carcinogenesis. Oncogene 26, 6448–6455 (2007). https://doi.org/10.1038/sj.onc.1210466

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