Abstract
The chicken c-mil/raf-1 gene (formerly also known as c-mht) was originally identified in the search for the cellular counterpart to the v-mil oncogene of the Mill Hill 2 retrovirus and was among the first cellular proto-oncogenes discovered. Although the c-mil/raf-1 promotor, as well as the exons transduced into v-mil, were characterized in detail, an entire map of this locus has never been published. Here, we now report the location of five previously unmapped exons. In addition, we have noticed inconsistent numbering of the c-mil/raf-1 exons in the literature and the GenBank database. Thus, we provide here a complete map of the c-mil/raf-1 gene and a revision of the exon numbers. Comparison of the chicken c-mil/raf-1 gene with those of other vertebrates suggests that the numbers and lengths of the translated exons of the raf-1 locus were established early in the vertebrate lineage and have been conserved during the divergent evolution of teleosts and tetrapods.
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Acknowledgements
We thank Drs Hassan Jumaa and Peter J Nielsen for helpful discussions and Gabrielle Siegers for critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft through SFB 388 and the Leibniz Prize to MR.
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Brummer, T., Stéhelin, D., Misawa, Y. et al. A revised and complete map of the chicken c-mil/raf-1 locus. Oncogene 23, 3128–3131 (2004). https://doi.org/10.1038/sj.onc.1207434
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DOI: https://doi.org/10.1038/sj.onc.1207434