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Stat3-dependent induction of BATF in M1 mouse myeloid leukemia cells

Oncogene volume 21, pages 8186–8191 (2002)Cite this article

Abstract

Stat3 mediates cellular responses associated with proliferation, survival and differentiation, but the mechanisms underlying the diverse effects of this signaling molecule remain unknown. M1 mouse myeloid leukemia cells arrest growth and differentiate into macrophages following treatment with interleukin 6 (IL-6) or leukemia inhibitory factor (LIF), and recent studies have shown that Stat3 plays a central role in this process. Utilizing representational difference analysis, we demonstrate that expression of the mouse BATF gene is upregulated as an early response to IL-6/LIF stimulation and Stat3 activation in this cell system. Immunoblots using antibodies to BATF detected an increase in BATF protein in response to LIF/IL-6 stimulation. BATF is a member of the AP-1 family of basic leucine zipper transcription factors and functions to inhibit the transcriptional and biological functions of AP-1 activity in mammalian cells. BATF forms complexes with c-Jun in M1 cells and forced expression of BATF in the absence of Stat3 signaling results in a reduced rate of cellular growth. These results indicate that Stat3 mediates cellular growth by modulating AP-1 activity through the induction of BATF.

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Acknowledgements

This work was supported by grants-in-aid for COE Research from the Ministry of Japan Society for promotion of Science and by PHS award CA78264 to EJ Taparowsky.

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Authors and Affiliations

  1. Department of Molecular Pathogenesis, Nagoya University School of Medicine, 65 Tsurumai Showa Nagoya 466-8550, Japan

    Takeshi Senga & Michinari Hamaguchi

  2. Radioisotope Research Center Medical Division, Nagoya University School of Medicine, 65 Tsurumai Showa Nagoya 466-8550, Japan

    Takashi Iwamoto

  3. Division of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-8640, Ishikawa, Japan

    Takashi Yokota

  4. Department of Biological Sciences, Purdue University, West Lafayette, 47907-1392, Indiana, IN, USA

    Sean E Humphrey & Elizabeth J Taparowsky

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  1. Takeshi Senga
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Correspondence to Michinari Hamaguchi.

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Senga, T., Iwamoto, T., Humphrey, S. et al. Stat3-dependent induction of BATF in M1 mouse myeloid leukemia cells. Oncogene 21, 8186–8191 (2002). https://doi.org/10.1038/sj.onc.1205918

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