Abstract
The human serine/threonine kinase Aurora-B is structurally related to the protein kinase Ipl1p from S cerevisiae and aurora from Drosophila melanogaster, which are key regulators of mitosis. The present study shows that human Aurora-B is activated by okadaic acid and forms complexes with the protein serine/threonine phosphatase type 1 (PP1) or PP2A, but not with PP5. These data identified Aurora-B associated protein phosphatases as negative regulators of kinase activation. We then used a series of substrates based on a histone H3 phosphorylation site (residues 5–15) to determine the substrate specificity of human Aurora-B. We found that this enzyme is an arginine-directed kinase that can phosphorylate histone H3 at serines 10 and 28 in vitro, suggesting that human Aurora-B is a mitotic histone H3 kinase.
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Acknowledgements
We thank Dr Tadashi Yamamoto (University of Tokyo, Tokyo) for pME-Gex4T-1 and Ms Tomoko Tsurutome for excellent technical assistance. This work was supported by grants-in-aid for Scientific Research and Core of Excellence from the Ministry of Education, Science, Sports and Culture of Japan.
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Sugiyama, K., Sugiura, K., Hara, T. et al. Aurora-B associated protein phosphatases as negative regulators of kinase activation. Oncogene 21, 3103–3111 (2002). https://doi.org/10.1038/sj.onc.1205432
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DOI: https://doi.org/10.1038/sj.onc.1205432
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