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  • Original Paper
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Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP

Oncogene volume 18, pages 1495–1501 (1999)Cite this article

Abstract

Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for β-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74 – 122 and the region spanning residues 1283 – 1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.

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Acknowledgements

We wish to thank Drs R Maki and RH Goodman for the plasmids of mouse PU.1 and CBP, Drs H Ariga and T Matsuyama for the positive control plasmids in yeast two-hybrid system. This research was supported by Grants-in-Aid for Specifical Project Research to TO (09254267 and 10152262) and for Encouragement of Young Scientists to HY (09770837) from the Ministry of Education, Science and Culture, Japan. We also thank financial support to YH by The Vehicle Racing Commemorative Foundation, Tokyo, and to TO by OB-GYN Akiyama Memorial Hospital, Hakodate, Japan.

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  1. Department of Cell Genetics, Sasaki Institute, 2-2, Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

    Hitomi Yamamoto, Fumiko Kihara-Negishi, Toshiyuki Yamada, Yoshiyuki Hashimoto & Tsuneyuki Oikawa

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  1. Hitomi Yamamoto
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Yamamoto, H., Kihara-Negishi, F., Yamada, T. et al. Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP. Oncogene 18, 1495–1501 (1999). https://doi.org/10.1038/sj.onc.1202427

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