Abstract
Dynamic structural changes of macromolecules undergoing biochemical reactions can be studied using novel single molecule spectroscopy tools. Recent advances in applying such distance and orientation molecular rulers to biological systems are reviewed, and future prospects and challenges are discussed.
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Acknowledgements
The author is indebted to A.P. Alivisatos, U. Banin, M. Bruchez, D.S. Chemla, M. Dahan, A.A. Deniz, Th. Enderle, J. Glass, J. Grunwell, T. Ha, T. Her, Th. Lacoste, T. Laurence, J. Liang, S. Licht, A. B. Martin, X. Michalet, F. Pinaud, P.G. Schultz, P.R. Selvin, A. Sonnleitner, and A.Y. Ting for their contributions to the single molecule spectroscopy effort in Berkeley and to A.A. Deniz, T. Her and X. Michalet for critical reading of the manuscript. This work was supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory and the Director, Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy and the Office of Naval Research.
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Weiss, S. Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy. Nat Struct Mol Biol 7, 724–729 (2000). https://doi.org/10.1038/78941
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DOI: https://doi.org/10.1038/78941
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