Perspectives
Nature Reviews Molecular Cell Biology 7, 449-456 (June 2006) | doi:10.1038/nrm1929
Innovation: Visualization of molecular interactions by fluorescence complementation
Tom K. Kerppola1 About the author
Abstract
The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.
Author affiliations
-
Tom K. Kerppola is at the Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA.
Email: kerppola@umich.edu
Published online 19 April 2006
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Single protein complex visualization: Seeing is believingNature Methods News and Views (01 Dec 2006)
Complementary methods for studies of protein interactions in living cellsNature Methods News and Views (01 Dec 2006)
See all 3 matches for News And ViewsRESEARCH
Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysisNature Biotechnology Research (01 May 2003)
RNA visualization in live bacterial cells using fluorescent protein complementationNature Methods Article (01 May 2007)
See all 21 matches for Research
