Abstract

Many efforts to determine microRNA (miRNA) targets have relied on computational approaches, which are hampered by high false positive rates because the target site sequences are short and occur often in the genome. This problem has been overcome by a recent study, which combines HITS-CLIP (high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation) with bioinformatics to produce a map of functionally relevant miRNA binding sites.