This webcast focuses on the use of primary cells in advanced 3D cell culture systems. Ideally, the cells used in cell culture systems should mimic those present in vivo, thus primary cells (non-immortalized cells that have recently been removed from an in vivo environment) are preferable to immortalized cells grown in cell culture for long periods. Given that cells grow in a 3D environment in vivo, alongside a variety of different cells types, cell culture systems that permit growth of multiple layers of various primary cell types in vitro are preferable to the 2D mono-culture methods traditionally used for cell culture. Some 3D cell culture systems are established by growing cells in scaffolds made from a variety of diverse materials, including collagen, hydrogels, decellularized ex vivo material and silk. Alternatively, microchips with microfluidic channels can be lined with cells to mimic the 3D microenvironment. Three researchers (Roger Kamm, David Kaplan and Harald Ott) describe the use of primary cells in some of these advanced 3D cell culture systems. The production of the webcast was supported by Lonza. As always, Nature Publishing Group retains sole responsibility for all editorial content.
Förster resonance energy transfer (FRET) can be used to detect and quantify interactions between two molecules, or two parts of a molecule. Fluorescence microscopy is commonly used to detect these interactions. FRET has been applied to a variety of cell types and can thus be used to address a diverse range of biological questions. In this webcast we briefly over-view the theoretical principles of FRET and discuss a range of applications in which it has been used. Experts with extensive experience using FRET discuss the practical considerations that need to be considered when embarking on a study using FRET, for example, selection of fluorophore pairs and minimisation of imaging-induced artefacts. In addition, as inspiration for your own future research, you can hear about some occasions in which the researchers have used FRET in particularly innovative ways. The webcast concludes with a live Q&A session with the speakers. The production of the webcast was supported by Olympus. As always, Nature Publishing Group retains sole responsibility for all editorial content.
Optogenetics allows highly targeted and fast control of biological processes in systems as complex as freely moving mammals and was named "Method of the Year" by Nature Methods in 2010. In this webcast two of the leading innovators of this rapidly developing approach, Professor Karl Deisseroth and Dr Michael Bruchas, talk about their individual approaches for employing optogenetics in their laboratories. It concentrates particularly on the challenges encountered when attempting to use optogenetics to study the neural bases for animal behaviour. The webcast concludes with a live Q&A session with the two speakers. The production of the webcast was supported by the National Science Foundation under Grant No. 1348284. As always, Nature Publishing Group retains sole responsibility for all editorial content.