Volume 17 Issue 4, April 2022

Organoids are increasingly used as model systems in precision medicine, but they are delicate, making it difficult to get accurate spatial chemical information. This protocol describes how to prepare organoid samples for mass spectrometry imaging.

[⁶⁸Ga]Ga-PSMA-11 has recently been approved by the US Food and Drug Administration as a radiopharmaceutical for PET imaging of prostate cancer. To meet higher demand for this tracer, procedures for its synthesis using both generator-eluted and cyclotron-produced ⁶⁸Ga are described.

The authors present a protocol for using the CRISPR–Cas9 genome editing system to knock out a gene of interest in human intestinal tissue–derived enteroids by lentiviral transduction and single-cell cloning.

Gorris et al. present the synthesis, biofunctionalization and purification of lanthanide-doped photon-upconversion nanoparticles and describe their application in both immunoassays for sensitive detection of blood-based biomarkers and bioimaging of cancer cells.

The authors present detailed procedures to fabricate, encapsulate and implant advanced wireless battery-free devices in head- and back-mounted forms in order to facilitate optogenetic experiments in freely moving rodents.

This protocol describes how to design genetic circuits using the web implementation of the Cello 2.0 software, which allows users to construct a DNA sequence for a genetic circuit composed of defined elements and predicts the circuit’s performance in new organisms and genetic contexts.

This protocol describes how to use the Binding Free-Energy Estimator 2 (BFEE2) software to calculate the standard binding free energy of a variety of protein:ligand complexes with different properties.

The authors provide protocols for rapid and scalable genome engineering in somatic cells of the liver and pancreas through both viral and nonviral delivery of CRISPR components into living mice.