Volume 13 Issue 8, August 2018

This protocol describes a proteomics approach for quantifying newly synthesized proteins (NSPs). NSPs are pulse-labeled with the methionine analog AHA or a heavy-isotope version of it, followed by AHA-peptide enrichment and LC-MS/MS analysis.

In this behavioral task, two objects are placed at specific locations at different times, and the response of mice and rats to these positions is assessed. The results indicate the rodents’ ability to recognize changes in spatial pattern separation.

This protocol describes the fabrication of AngioChip, a microfabricated scaffold for engineering vascularized tissues. In addition, the protocol contains procedures for immunostaining, drug screening and surgical anastomosis of the AngioChip.

This protocol describes a pig model in which lungs are transplanted into recipients who are subsequently monitored for 3 d after surgery to study ischemia reperfusion injury and early graft function.

HyperTRIBE uses a hyperactive RNA-editing enzyme fused to an RNA-binding protein (RBP) to mark the target RNA transcripts of the RBP by converting adenosine to inosine near the binding sites with increased efficiency and reduced sequence bias.

This protocol describes a noninvasive approach to evoke microglial engulfment and reduce amyloid levels in mouse brain. The authors describe assembly and operation of a light-flicker system, as well as assessment of the molecular and cellular effects.

This protocol describes how to combine expansion microscopy (ExM) with structured illumination microscopy (SIM). ExM–SIM is exemplified by super-resolution analysis of the synaptonemal complex (SC) and single-particle averaging of SC proteins.