Volume 10 Issue 3, March 2015

This protocol describes how in-solution protein FTIR can be used to obtain information about the relative contributions of α-helices, β-sheets, β-turn, and random coil structures to a protein's secondary structure.

This protocol describes how to achieve optogenetics- and imaging-aided physiological analysis of synaptic interconnections among 4–30 simultaneously and sequentially recorded neurons.

This protocol describes how to direct differentiation of human pluripotent stem cells into developmental lung progenitors, and subsequently into predominantly distal lung epithelial cells, by following four stages that recapitulate lung development.

Targeted proteomics by SRM/MRM or SWATH MS relies on reference assay libraries for peptide identification. This protocol is for building extensive, high-quality reference libraries starting with data acquired by discovery proteomics.

Lee et al. provide their protocol for fluorescent in situ sequencing (FISSEQ) of RNA. This technique allows spatial and quantitative information about mRNA expression to be obtained simultaneously.

In this protocol, the authors describe an approach for PCA-based 3D reconstruction of hollow sphere–shaped tissues and organs from single-cell gene expression data.

This protocol describes how to prepare a sequencing library for MethylC-seq analysis, enabling the methylation status of cytosines in genomic DNA to be determined at nucleotide resolution on a genome-wide scale.

With this protocol rat livers can be kept viable for up to 96 h and transplanted successfully. The livers are first loaded with cryoprotectants to prevent ice formation and protect against hypothermic injury, and they are then cooled to –6 °C without freezing.

This protocol describes mouse and rat models of Roux-en-Y gastric bypass and sleeve gastrectomy, which are similar to bariatric operations performed to treat obese humans.

Sortase A can be used for the site-specific modification of proteins. This protocol describes its immobilization on Sepharose beads, allowing for larger-scale continuous flow reactions or easy separation from the enzyme in batch reactions.

This protocol enables the statistical analysis of cell motility in 2D and 3D microenvironments using the anisotropic persistent random walk model, providing a new tool to characterize 3D cell migration.

There is a growing demand for renewable transportation fuels. This protocol describesthe production of an acetone-butanol-ethanol mixture in Clostridiumacetobutylicum as well as its reaction to form long-chain ketones for testing as car or jet fuel.