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Protocols

Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels pp1247 - 1259

Yoojin Shin, Sewoon Han, Jessie S Jeon, Kyoko Yamamoto, Ioannis K Zervantonakis, Ryo Sudo, Roger D Kamm & Seok Chung

doi:10.1038/nprot.2012.051

Subject terms: Cell biology | Cell culture | Nanotechnology


A post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs pp1260 - 1284

Martin T Swain, Isheng J Tsai, Samual A Assefa, Chris Newbold, Matthew Berriman & Thomas D Otto

doi:10.1038/nprot.2012.068

Subject terms: Computational biology | Genomics


Determination of site-specific glycan heterogeneity on glycoproteins pp1285 - 1298

Daniel Kolarich, Pia H Jensen, Friedrich Altmann & Nicolle H Packer

doi:10.1038/nprot.2012.062

Subject terms: Protein analysis | Proteomics | Spectroscopy


Structural analysis of N- and O-glycans released from glycoproteins pp1299 - 1310

Pia H Jensen, Niclas G Karlsson, Daniel Kolarich & Nicolle H Packer

doi:10.1038/nprot.2012.063

Subject terms: Protein analysis | Proteomics | Spectroscopy




Sensitive detection of chromatin coassociations using enhanced chromosome conformation capture on chip pp1335 - 1350

Tom Sexton, Sreenivasulu Kurukuti, Jennifer A Mitchell, David Umlauf, Takashi Nagano & Peter Fraser

doi:10.1038/nprot.2012.071

Subject terms: Epigenomics | Genetic analysis


Analysis of neurotransmitter release mechanisms by photolysis of caged Ca2+ in an autaptic neuron culture system pp1351 - 1365

Andrea Burgalossi, SangYong Jung, Kwun-nok Mimi Man, Ramya Nair, Wolf J Jockusch, Sonja M Wojcik, Nils Brose & Jeong-Seop Rhee

doi:10.1038/nprot.2012.074

Subject terms: Cell culture | Neuroscience


Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product–free environment pp1366 - 1381

Emma Stephenson, Laureen Jacquet, Cristian Miere, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Yaser Dajani, Peter Braude & Dusko Ilic

doi:10.1038/nprot.2012.080

Subject term: Cell culture



Protein misfolding cyclic amplification of infectious prions pp1397 - 1409

Rodrigo Morales, Claudia Duran-Aniotz, Rodrigo Diaz-Espinoza, Manuel V Camacho & Claudio Soto

doi:10.1038/nprot.2012.067

Subject terms: Neuroscience | Protein analysis




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