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Protocols

Highly multiplexed and strand-specific single-cell RNA 5′ end sequencing pp813 - 828

Saiful Islam, Una Kjällquist, Annalena Moliner, Pawel Zajac, Jian-Bing Fan, Peter Lönnerberg & Sten Linnarsson

doi:10.1038/nprot.2012.022

Subject terms: Genomics | Nucleic acid based molecular biology



RGB marking with lentiviral vectors for multicolor clonal cell tracking pp839 - 849

Kristoffer Weber, Michael Thomaschewski, Daniel Benten & Boris Fehse

doi:10.1038/nprot.2012.026

Subject terms: Cell biology | Cell culture | Genetic modification | Imaging


Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro pp850 - 858

Yuji Haraguchi, Tatsuya Shimizu, Tadashi Sasagawa, Hidekazu Sekine, Katsuhisa Sakaguchi, Tetsutaro Kikuchi, Waki Sekine, Sachiko Sekiya, Masayuki Yamato, Mitsuo Umezu & Teruo Okano

doi:10.1038/nprot.2012.027

Subject terms: Cell biology | Cell culture | Model organisms | Pharmacology | Tissue culture | Toxicology





Parallel detection of antigen-specific T cell responses by combinatorial encoding of MHC multimers pp891 - 902

Rikke Sick Andersen, Pia Kvistborg, Thomas Mørch Frøsig, Natasja W Pedersen, Rikke Lyngaa, Arnold H Bakker, Chengyi Jenny Shu, Per thor Straten, Ton N Schumacher & Sine Reker Hadrup

doi:10.1038/nprot.2012.037

Subject terms: Cell biology | Immunological techniques | Isolation, purification and separation | Isolation, purification and separation



A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins pp921 - 934

Jiajie Diao, Yuji Ishitsuka, Hanki Lee, Chirlmin Joo, Zengliu Su, Salman Syed, Yeon-Kyun Shin, Tae-Young Yoon & Taekjip Ha

doi:10.1038/nprot.2012.020

Subject terms: Biochemistry | Cell biology | Imaging | Neuroscience



Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila pp946 - 958

Helena M Cochemé, Angela Logan, Tracy A Prime, Irina Abakumova, Caroline Quin, Stephen J McQuaker, Jigna V Patel, Ian M Fearnley, Andrew M James, Carolyn M Porteous, Robin A J Smith, Richard C Hartley, Linda Partridge & Michael P Murphy

doi:10.1038/nprot.2012.035

Subject terms: Biochemistry | Cell biology | Model organisms | Spectroscopy




Cell type–specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP pp978 - 994

Stefan Bonn, Robert P Zinzen, Alexis Perez-Gonzalez, Andrew Riddell, Anne-Claude Gavin & Eileen E M Furlong

doi:10.1038/nprot.2012.049

Subject terms: Epigenomics | Genomics | Immunological techniques



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