Box 1. Protocol for preparation of electrocompetent JUDE-1 E. coli cells carrying plasmid pBAD33-NlpA-ZZ

From the following article

E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display

Yariv Mazor, Thomas Van Blarcom, Brent L Iverson & George Georgiou

Nature Protocols 3, 1766 - 1777 (2008) Published online: 23 October 2008

doi:10.1038/nprot.2008.176

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E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display