Protocol abstract
Nature Protocols 2, - 141 - 151 (2007)
Published online: 15 February 2007 | doi:10.1038/nprot.2006.342
Subject Categories: Neuroscience | Model organisms | Pharmacology and toxicology
Protocol for the MPTP mouse model of Parkinson's disease
Vernice Jackson-Lewis1 & Serge Przedborski1,2
Abstract
This protocol describes our method of producing a reliable mouse model of Parkinson's disease (PD) using the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We discuss the particulars of the model, provide key references and outline what investigators need to know to develop the MPTP mouse model of PD safely and successfully. Completion of this protocol depends on the regimen of MPTP used and on the actual planned studies, which often range from 7 to 30 d. This protocol calls for implementation of safety measures and for the acquisition of several pieces of equipment, which are a one-time investment worth making if one elects to use this model on a regular basis.
- Department of Neurology, Columbia University, New York, NY 10032, USA.
- Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
Correspondence to: Serge Przedborski1,2 e-mail: SP30@Columbia.edu
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