Volume 15 Issue 12, December 2020

This tutorial describes how to design nanoparticle-based LFAs for detecting biomolecules. The authors provide guidance on how to select the appropriate lateral-flow strip components and bioreceptors as well as detection strategies.

Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

This extension of the original silicon fluoride acceptor (SiFA) protocol for ¹⁸F-labeling of peptides describes modifications required for the preparation of clinical-grade [¹⁸F]SiFAlin-TATE for diagnosis of neuroendocrine tumors via PET imaging.

This protocol describes a comet-based in vitro assay for detecting base and nucleotide excision repair activity for use in cell culture model systems, human biomonitoring and clinical investigations, and animal studies, using isolated blood cells and various solid tissues.

Label-free and reagentless analysis of biomarkers is ideal for point-of-care diagnostics. In this protocol, changes in electrochemical capacitance resulting from biomarker binding to an electrode coated with a biological receptor are detected.

This protocol describes experimental and computational procedures for genome-wide detection of endogenous and induced DNA double-strand breaks (DSBs) in any cell type or tissue that can be brought into suspension.

Footprinting reports solvent accessibilities of a protein or protein complex. This protocol describes a fast photochemical oxidation of proteins platform for footprinting, with laser-initiated H₂O₂ photolysis and mass spectrometry analysis.

This protocol describes proximity labeling approaches using TurboID and split-TurboID, which can be used for mapping protein–protein interactions and organelle proteomes in live mammalian cells with nanometer spatial resolution.

The authors describe procedures for generating and biobanking glioblastoma organoids from patient tumor tissue and testing of chimeric antigen receptor T cell efficacy by co-culture. Tissue processing, immunohistology and detection of hypoxic gradients and actively proliferating cells are also described.

The authors describe a protocol for a high-throughput version of the MALDI-TOF–based deubiquitylating enzyme assay method.

This protocol describes experimental and computational procedures for obtaining genome-wide DNA replication timing maps based on copy-number differences derived from whole-genome amplification and next-generation sequencing of genomic DNA from single S-phase cells.

This phage-assisted continuous evolution protocol enables directed protein evolution. It includes preparation of selection phage and host cells, assembly of a continuous flow apparatus and performance and analysis of evolution experiments.