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Clusters of the main immune cell populations in the naive mouse brain compartment, including the parenchyma, meninges and choroid plexus. This SPADE plot depicts the various populations with color gradient indicating the median intensity of the CD45 marker. Identification of specific populations was performed based on the expression of multiple markers, as indicated for each cell type. Image taken from the protocol by Korin et al. doi:10.1038/nprot.2017.155. Cover design by Jamel Wooten.
Colonoscopy-guided mucosal injection is used to induce site-directed tumors and to transplant tumors into the distal colons of mice. Tumors for engraftment are obtained from cancer organoids derived from mouse or human tissue and can be genetically modified before use.
In this protocol, mouse or human colorectal cancer organoids are transplanted into the cecal epithelium. The transplanted organoids grow into a single primary tumor mass within the intestinal tract and tumor cells are capable of metastasis.
This protocol uses a three-layer system to organize rat primary testicular cells into organoids that can both establish and maintain germ cells in an environment containing a functional blood–testis barrier.
This protocol describes procedures for the high-throughput in situ X-ray screening of and data collection from soluble and membrane-protein crystals at room temperature and under cryogenic conditions using Mylar-based film sandwich plates.
This NeuCode SILAC/SILAM protocol uses neutron-coded lysine derivatives to improve stable isotope labeling of amino acids in cells or mice, enabling multiplexed comparison of proteomes from different samples by mass spectrometry–based proteomics.
This protocol provides a detailed workflow showing how CRISPR screens can be applied to the parasite Toxoplasma gondii. The method can be used to study how culture conditions affect genetic requirements of the parasite and to unravel genetic interactions.
Organozinc reagents are useful building blocks for introducing C(sp2)-C(sp3) and C(sp3)-C(sp3) bonds into organic compounds. This protocol describes a flow chemistry approach with which alkyl zinc derivatives can be stably prepared for use in downstream synthesis.
This protocol describes how to generate and use dendritic cells and combinatorial adjuvants to modulate tumor microenvironment and increase T-cell accumulation in tumors in vivo.
This protocol describes the use of Cas9 ribonucleoproteins and AAV6 donor templates to enable precise genome editing of human hematopoietic stem cells by homologous recombination (HR), including in vitro and in vivo assays for determining HR frequencies in HSPCs.
This protocol describes how to characterize immune cells in the mouse brain compartment using mass cytometry (CyTOF) and provides a step-by-step description from brain dissection to data analysis.
This protocol describes the heat-induced radiolabeling of Feraheme (FH) nanoparticles for PET/SPECT imaging and labeling of FH nanoparticles with fluorescent Cy5.5 dyes for flow cytometry.