Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
This Perspective discusses the development and applications of light-sheet-based fluorescence microscopy, a method with considerable advantages over confocal fluorescence microscopy.
This protocol describes a substantially redeveloped approach for the localization of organelle proteins by isotope tagging (LOPIT) to enable subcellular localization of thousands of proteins per experiment (hyperLOPIT).
This protocol describes how to characterize mouse retinal mononuclear phagocytes in vivo and in situ using scanning laser ophthalmoscopy of microglia, quantitative analysis of Iba1-stained retinal sections, CD11b-based retinal flow cytometry, and qRT–PCR analysis of key microglia markers.
Strand-seq is a single-cell template strand sequencing technology that generates directional genomic libraries, which allows the characterization of individual homologs.
This protocol describes how to generate defined embryoid bodies and subsequent standardized beating engineered heart tissue from human iPSCs using small molecules.
In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.
The comprehensive study of protein glycosylation has been complicated by the complex structural diversity of glycans. In this protocol, Yang et al. describe a solid-phase method for the sequential analysis of N-linked and O-linked glycans.
FISH-Flow is a flow-cytometry-based protocol enabling simultaneous mRNA and protein measurements in single nonadherent mammalian cells. The authors provide both a manual and a semiautomated, single-tube version of the protocol.
This multiplex PCR enrichment protocol enables sequencing of Zika and other viral genomes of low abundance from clinical samples using the Illumina platform, or the portable MinION sequencer, facilitating direct application in field situations.
Aziridine-containing cyclic tetrapeptides possess great potential for covalent protein labeling and can be valuable in screening libraries. Chung et al. describe the synthesis, cyclization, and site-specific modification of these scaffolds.