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Nature Protocols 1, 2759–2762 (1 December 2006) | doi:10.1038/nprot.2006.399

Use of a commercially available reagent for the selective detection of homocysteine in plasma

Jorge O Escobedo , Weihua Wang & Robert M Strongin

A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes |[sim]|30 min.