Protocol abstract
Nature Protocols 1, - 2759 - 2762 (2006)
Published online: 18 January 2007 | doi:10.1038/nprot.2006.399
Subject Categories: Chemical modification | Pharmacology and toxicology
Use of a commercially available reagent for the selective detection of homocysteine in plasma
Jorge O Escobedo1, Weihua Wang1 & Robert M Strongin1
Abstract
A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes 
30 min.
- Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Correspondence to: Robert M Strongin1 e-mail: rstrong@lsu.edu
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is an online service detailing information about specific products used in this article. You can view the product descriptions, request information and compare with other similar products. The products used are listed in alphabetical order.
A-Z product listing
- Certified buffer solution(Fisher)
- DL-Hcy(Sigma-Aldrich)
- Glass microsyringe(Hamilton)
- Glass vials(Gerresheimer)
- Hot plate, type 2300(Barnstead/Thermolyne)
- Human plasma(Sigma-Aldrich)
